Bacterial microcompartment-mediated ethanolamine metabolism in Escherichia coli urinary tract infection

Research output: Contribution to journalArticle

Authors

  • Katherine Dadswell
  • Sinead Creagh
  • Edward McCullagh
  • Mingzhi Liang
  • Ian R Brown
  • Martin J Warren
  • John MacSharry
  • Michael B Prentice

Colleges, School and Institutes

External organisations

  • School of Microbiology, University College Cork, Cork, Ireland.
  • Department of Microbiology, Cork University Hospital, Cork, Ireland.
  • School of Biosciences, University of Kent, Ingram Building, Canterbury CT2 7NJ, U.K.
  • Institute of Microbiology and Infection, College of Medical and Dental Science, University of Birmingham, Birmingham, UK.
  • APC Microbiome Ireland, University College Cork, Cork, Ireland.
  • Department of Pathology, University College Cork, Cork, Ireland.

Abstract

Urinary tract infections (UTIs) are common and in general are caused by intestinal uropathogenic Escherichia coli (UPEC) ascending via the urethra. Microcompartment- mediated catabolism of ethanolamine, a host cell breakdown product, fuels the competitive overgrowth of intestinal E. coli, both pathogenic enterohemorrhagic E. coli and commensal strains. During a UTI, urease-negative E. coli bacteria thrive, despite the comparative nutrient limitation in urine. The role of ethanolamine as a potential nutrient source during UTIs is understudied. We evaluated the role of the metabolism of ethanolamine as a potential nitrogen and carbon source for UPEC in the urinary tract. We analyzed infected urine samples by culture, highperformance liquid chromatography, reverse transcription-quantitative PCR, and genomic sequencing. The ethanolamine concentration in urine was comparable to the concentration of the most abundant reported urinary amino acid, D-serine. Transcription of the eut operon was detected in the majority of urine samples containing E. coli screened. All sequenced UPEC strains had conserved eut operons, while metabolic genotypes previously associated with UTI (dsdCXA, metE) were mainly limited to phylogroup B2. In vitro ethanolamine was found to be utilized as a sole source of nitrogen by UPEC strains. The metabolism of ethanolamine in artificial urine medium (AUM) induced metabolosome formation and provided a growth advantage at the physiological levels found in urine. Interestingly, eutE (which encodes acetaldehyde dehydrogenase) was required for UPEC strains to utilize ethanolamine to gain a growth advantage in AUM, suggesting that ethanolamine is also utilized as a carbon source. These data suggest that urinary ethanolamine is a significant additional carbon and nitrogen source for infecting E. coli strains.

Bibliographic note

Copyright © 2019 Dadswell et al.

Details

Original languageEnglish
Article numbere00211
JournalInfection and Immunity
Volume87
Issue number8
Early online date28 May 2019
Publication statusPublished - Aug 2019

Keywords

  • Escherichia coli, Ethanolamine, Metabolosome, Microcompartment, Urinary tract infection