Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling

Research output: Contribution to journalArticle

Authors

  • E Peter Magennis
  • Cheng Sui
  • Sebastian G Spain
  • David J Bradshaw
  • David Churchley
  • Giuseppe Mantovani
  • Klaus Winzer
  • Cameron Alexander

Colleges, School and Institutes

External organisations

  • University of Nottingham
  • GlaxoSmithKline, St Georges Avenue Weybridge KT13 0DE, Surrey, UK.

Abstract

The detection and inactivation of pathogenic strains of bacteria continues to be an important therapeutic goal. Hence, there is a need for materials that can bind selectively to specific microorganisms for diagnostic or anti-infective applications, but that can be formed from simple and inexpensive building blocks. Here, we exploit bacterial redox systems to induce a copper-mediated radical polymerization of synthetic monomers at cell surfaces, generating polymers in situ that bind strongly to the microorganisms that produced them. This 'bacteria-instructed synthesis' can be carried out with a variety of microbial strains, and we show that the polymers produced are self-selective binding agents for the 'instructing' cell types. We further expand on the bacterial redox chemistries to 'click' fluorescent reporters onto polymers directly at the surfaces of a range of clinical isolate strains, allowing rapid, facile and simultaneous binding and visualization of pathogens.

Details

Original languageEnglish
Pages (from-to)748-755
JournalNature Materials
Volume13
Issue number7
Publication statusPublished - 11 May 2014