Axon growth and guidance genes identify T-dependent germinal centre B cells

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Axon growth and guidance genes identify T-dependent germinal centre B cells. / Yu, D; Cook, MC; Shin, DM; Silva, DG; Marshall, Jennifer; Toellner, Kai-Michael; Havran, WL; Caroni, P; Cooke, MP; Morse, HC; MacLennan, Ian; Goodnow, CC; Vinuesa, CG.

In: Immunology and Cell Biology, Vol. 86, No. 1, 16.10.2007, p. 3-14.

Research output: Contribution to journalArticle

Harvard

Yu, D, Cook, MC, Shin, DM, Silva, DG, Marshall, J, Toellner, K-M, Havran, WL, Caroni, P, Cooke, MP, Morse, HC, MacLennan, I, Goodnow, CC & Vinuesa, CG 2007, 'Axon growth and guidance genes identify T-dependent germinal centre B cells', Immunology and Cell Biology, vol. 86, no. 1, pp. 3-14. https://doi.org/10.1038/sj.icb.7100123

APA

Yu, D., Cook, MC., Shin, DM., Silva, DG., Marshall, J., Toellner, K-M., Havran, WL., Caroni, P., Cooke, MP., Morse, HC., MacLennan, I., Goodnow, CC., & Vinuesa, CG. (2007). Axon growth and guidance genes identify T-dependent germinal centre B cells. Immunology and Cell Biology, 86(1), 3-14. https://doi.org/10.1038/sj.icb.7100123

Vancouver

Author

Yu, D ; Cook, MC ; Shin, DM ; Silva, DG ; Marshall, Jennifer ; Toellner, Kai-Michael ; Havran, WL ; Caroni, P ; Cooke, MP ; Morse, HC ; MacLennan, Ian ; Goodnow, CC ; Vinuesa, CG. / Axon growth and guidance genes identify T-dependent germinal centre B cells. In: Immunology and Cell Biology. 2007 ; Vol. 86, No. 1. pp. 3-14.

Bibtex

@article{ef75c597cc5949b2a98ebbf448b7e4ac,
title = "Axon growth and guidance genes identify T-dependent germinal centre B cells",
abstract = "Selection of B cells subjected to hypermutation in germinal centres (GC) during T cell-dependent (TD) antibody responses yields memory cells and long-lived plasma cells that produce high affinity antibodies biased to foreign antigens rather than self-antigens. GC also form in T-independent (TI) responses to polysaccharide antigens but failed selection results in GC involution and memory cells are not generated. To date there are no markers that allow phenotypic distinction of T-dependent and TI germinal centre B cells. We compared the global gene expression of GC B cells purified from mice immunized with either TD or TI antigens and identified eighty genes that are differentially expressed in TD GC. Significantly, the largest cluster comprises genes involved in growth and guidance of neuron axons such as Plexin B2, Basp1, Nelf, Shh, Sc4mol and Sult4alpha. This is consistent with formation of long neurite (axon and dendrite)-like structures by mouse and human GC B cells, which may facilitate T:B cell interactions within GC, affinity maturation and B cell memory formation. Expression of BASP1 and PLEXIN B2 protein is very low or undetectable in resting and TI GC B cells, but markedly upregulated in GC B cells induced in the presence of T cell help. Finally we show some of the axon growth genes upregulated in TD-GC B cells including Basp1, Shh, Sult4alpha, Sc4mol are also preferentially expressed in post-GC B cell neoplasms.",
keywords = "B cell memory, axon, T-dependent, germinal centre",
author = "D Yu and MC Cook and DM Shin and DG Silva and Jennifer Marshall and Kai-Michael Toellner and WL Havran and P Caroni and MP Cooke and HC Morse and Ian MacLennan and CC Goodnow and CG Vinuesa",
year = "2007",
month = oct,
day = "16",
doi = "10.1038/sj.icb.7100123",
language = "English",
volume = "86",
pages = "3--14",
journal = "Immunology and Cell Biology",
issn = "0818-9641",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Axon growth and guidance genes identify T-dependent germinal centre B cells

AU - Yu, D

AU - Cook, MC

AU - Shin, DM

AU - Silva, DG

AU - Marshall, Jennifer

AU - Toellner, Kai-Michael

AU - Havran, WL

AU - Caroni, P

AU - Cooke, MP

AU - Morse, HC

AU - MacLennan, Ian

AU - Goodnow, CC

AU - Vinuesa, CG

PY - 2007/10/16

Y1 - 2007/10/16

N2 - Selection of B cells subjected to hypermutation in germinal centres (GC) during T cell-dependent (TD) antibody responses yields memory cells and long-lived plasma cells that produce high affinity antibodies biased to foreign antigens rather than self-antigens. GC also form in T-independent (TI) responses to polysaccharide antigens but failed selection results in GC involution and memory cells are not generated. To date there are no markers that allow phenotypic distinction of T-dependent and TI germinal centre B cells. We compared the global gene expression of GC B cells purified from mice immunized with either TD or TI antigens and identified eighty genes that are differentially expressed in TD GC. Significantly, the largest cluster comprises genes involved in growth and guidance of neuron axons such as Plexin B2, Basp1, Nelf, Shh, Sc4mol and Sult4alpha. This is consistent with formation of long neurite (axon and dendrite)-like structures by mouse and human GC B cells, which may facilitate T:B cell interactions within GC, affinity maturation and B cell memory formation. Expression of BASP1 and PLEXIN B2 protein is very low or undetectable in resting and TI GC B cells, but markedly upregulated in GC B cells induced in the presence of T cell help. Finally we show some of the axon growth genes upregulated in TD-GC B cells including Basp1, Shh, Sult4alpha, Sc4mol are also preferentially expressed in post-GC B cell neoplasms.

AB - Selection of B cells subjected to hypermutation in germinal centres (GC) during T cell-dependent (TD) antibody responses yields memory cells and long-lived plasma cells that produce high affinity antibodies biased to foreign antigens rather than self-antigens. GC also form in T-independent (TI) responses to polysaccharide antigens but failed selection results in GC involution and memory cells are not generated. To date there are no markers that allow phenotypic distinction of T-dependent and TI germinal centre B cells. We compared the global gene expression of GC B cells purified from mice immunized with either TD or TI antigens and identified eighty genes that are differentially expressed in TD GC. Significantly, the largest cluster comprises genes involved in growth and guidance of neuron axons such as Plexin B2, Basp1, Nelf, Shh, Sc4mol and Sult4alpha. This is consistent with formation of long neurite (axon and dendrite)-like structures by mouse and human GC B cells, which may facilitate T:B cell interactions within GC, affinity maturation and B cell memory formation. Expression of BASP1 and PLEXIN B2 protein is very low or undetectable in resting and TI GC B cells, but markedly upregulated in GC B cells induced in the presence of T cell help. Finally we show some of the axon growth genes upregulated in TD-GC B cells including Basp1, Shh, Sult4alpha, Sc4mol are also preferentially expressed in post-GC B cell neoplasms.

KW - B cell memory

KW - axon

KW - T-dependent

KW - germinal centre

U2 - 10.1038/sj.icb.7100123

DO - 10.1038/sj.icb.7100123

M3 - Article

C2 - 17938642

VL - 86

SP - 3

EP - 14

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

SN - 0818-9641

IS - 1

ER -