Automatic exposure control
and estimation of effective system noise
in diffuse fluorescence tomography

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Automatic exposure control
and estimation of effective system noise
in diffuse fluorescence tomography. / Kepshire, DL; Dehghani, Hamid; Leblond, F; Pogue, BW.

In: Optics Express, Vol. 17, No. 25, 01.01.2009, p. 23272-23283.

Research output: Contribution to journalArticle

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@article{5632da175c6347c6b4c5636113e8fdfc,
title = "Automatic exposure control
and estimation of effective system noise
in diffuse fluorescence tomography",
abstract = "A diffuse fluorescence tomography system, based upon time-correlated single photon counting, is presented with an automated algorithm to allow dynamic range variation through exposure control. This automated exposure control allows the upper and lower detection levels of fluorophore to be extended by an order of magnitude beyond the previously published performance and benefits in a slight decrease in system effective noise. The effective noise level is used as a metric to characterize the system performance, integrating both model-mismatch and calibration bias errors into a single parameter. This effective error is near 7% of the reconstructed fluorescent yield value, when imaging in just few minutes. Quantifying protoporphyrin IX concentrations down to 50 ng/ml is possible, for tumor-sized regions. This fluorophore has very low fluorescence yield, but high biological relevance for tumor imaging, given that it is produced in the mitochondria, and upregulated in many tumor types. (C) 2009 Optical Society of America",
keywords = "Collagen structure, ECM (extracellular matrix), Cartilage, Elasticity, Mechanical properties",
author = "DL Kepshire and Hamid Dehghani and F Leblond and BW Pogue",
year = "2009",
month = jan,
day = "1",
doi = "10.1364/OE.17.023272",
language = "English",
volume = "17",
pages = "23272--23283",
journal = "Optics Express",
issn = "1094-4087",
publisher = "Optical Society of America",
number = "25",

}

RIS

TY - JOUR

T1 - Automatic exposure control
and estimation of effective system noise
in diffuse fluorescence tomography

AU - Kepshire, DL

AU - Dehghani, Hamid

AU - Leblond, F

AU - Pogue, BW

PY - 2009/1/1

Y1 - 2009/1/1

N2 - A diffuse fluorescence tomography system, based upon time-correlated single photon counting, is presented with an automated algorithm to allow dynamic range variation through exposure control. This automated exposure control allows the upper and lower detection levels of fluorophore to be extended by an order of magnitude beyond the previously published performance and benefits in a slight decrease in system effective noise. The effective noise level is used as a metric to characterize the system performance, integrating both model-mismatch and calibration bias errors into a single parameter. This effective error is near 7% of the reconstructed fluorescent yield value, when imaging in just few minutes. Quantifying protoporphyrin IX concentrations down to 50 ng/ml is possible, for tumor-sized regions. This fluorophore has very low fluorescence yield, but high biological relevance for tumor imaging, given that it is produced in the mitochondria, and upregulated in many tumor types. (C) 2009 Optical Society of America

AB - A diffuse fluorescence tomography system, based upon time-correlated single photon counting, is presented with an automated algorithm to allow dynamic range variation through exposure control. This automated exposure control allows the upper and lower detection levels of fluorophore to be extended by an order of magnitude beyond the previously published performance and benefits in a slight decrease in system effective noise. The effective noise level is used as a metric to characterize the system performance, integrating both model-mismatch and calibration bias errors into a single parameter. This effective error is near 7% of the reconstructed fluorescent yield value, when imaging in just few minutes. Quantifying protoporphyrin IX concentrations down to 50 ng/ml is possible, for tumor-sized regions. This fluorophore has very low fluorescence yield, but high biological relevance for tumor imaging, given that it is produced in the mitochondria, and upregulated in many tumor types. (C) 2009 Optical Society of America

KW - Collagen structure

KW - ECM (extracellular matrix)

KW - Cartilage

KW - Elasticity

KW - Mechanical properties

U2 - 10.1364/OE.17.023272

DO - 10.1364/OE.17.023272

M3 - Article

C2 - 20052253

VL - 17

SP - 23272

EP - 23283

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 25

ER -