AtMYB93 is a novel negative regulator of lateral root development in Arabidopsis

Research output: Contribution to journalArticle

Authors

  • Ute Voss
  • Susan Harding
  • Jessica Fannon
  • Erika Yamada
  • Kamal Swarup
  • Anushree Choudhary
  • Julien Lavenus
  • Susan Bradshaw
  • Malcolm J Bennett
  • Juliet Coates

Colleges, School and Institutes

External organisations

  • University of Nottingham
  • University of Birmingham, School of Biosciences, UK

Abstract

Plant root system plasticity is critical for survival in changing environmental conditions. One important aspect of root architecture is lateral root development, a complex process regulated by hormone, environmental and protein signalling pathways.
Here we show, using molecular genetic approaches, that the MYB transcription factor AtMYB93 is a novel negative regulator of lateral root development in Arabidopsis.
We identify AtMYB93 as an interaction partner of the lateral-root-promoting ARABIDILLO proteins. Atmyb93 mutants have faster lateral root developmental progression and enhanced lateral root densities, while AtMYB93-overexpressing lines display the opposite phenotype. AtMYB93 is expressed strongly, specifically and transiently in the endodermal cells overlying early lateral root primordia and is additionally induced by auxin in the basal meristem of the primary root. Furthermore, Atmyb93 mutant lateral root development is insensitive to auxin, indicating that AtMYB93 is required for normal auxin responses during lateral root development.
We propose that AtMYB93 is part of a novel auxin-induced negative feedback loop stimulated in a select few endodermal cells early during lateral root development, ensuring that lateral roots only develop when absolutely required. Putative AtMYB93 homologues are detected throughout flowering plants and represent promising targets for manipulation.

Details

Original languageEnglish
Pages (from-to)1194–1207
Number of pages14
JournalNew Phytologist
Volume203
Issue number4
Early online date6 Jun 2014
Publication statusPublished - Sep 2014

Keywords

  • abscisic acid, endodermis, lateral roots, MYB, negative regulation, auxin, ARABIDILLO