Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases

Research output: Contribution to journalArticlepeer-review

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Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases. / Lindner, Diana; Fitzek, Antonia; Brauninger, Hanna; Aleshcheva, Ganna; Edler, Caroline; Meissner, Kira; Scherschel, Katharina; Kirchhof, Paulus; Escher, Felicitas; Schultheiss, Heinz-Peter; Blankenberg, Stefan S; Puschel, Klaus; Westermann, Dirk.

In: JAMA Cardiology, 27.07.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Lindner, D, Fitzek, A, Brauninger, H, Aleshcheva, G, Edler, C, Meissner, K, Scherschel, K, Kirchhof, P, Escher, F, Schultheiss, H-P, Blankenberg, SS, Puschel, K & Westermann, D 2020, 'Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases', JAMA Cardiology. https://doi.org/10.1001/jamacardio.2020.3551

APA

Lindner, D., Fitzek, A., Brauninger, H., Aleshcheva, G., Edler, C., Meissner, K., Scherschel, K., Kirchhof, P., Escher, F., Schultheiss, H-P., Blankenberg, S. S., Puschel, K., & Westermann, D. (2020). Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases. JAMA Cardiology. https://doi.org/10.1001/jamacardio.2020.3551

Vancouver

Author

Lindner, Diana ; Fitzek, Antonia ; Brauninger, Hanna ; Aleshcheva, Ganna ; Edler, Caroline ; Meissner, Kira ; Scherschel, Katharina ; Kirchhof, Paulus ; Escher, Felicitas ; Schultheiss, Heinz-Peter ; Blankenberg, Stefan S ; Puschel, Klaus ; Westermann, Dirk. / Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases. In: JAMA Cardiology. 2020.

Bibtex

@article{6e6005d4bb4c4056a5a31a32a80a82ac,
title = "Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases",
abstract = "Importance: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be documented in various tissues, but the frequency of cardiac involvement as well as possible consequences are unknown. Objective: To evaluate the presence of SARS-CoV-2 in the myocardial tissue from autopsy cases and to document a possible cardiac response to that infection. Design, Setting, and Participants: This cohort study used data from consecutive autopsy cases from Germany between April 8 and April 18, 2020. All patients had tested positive for SARS-CoV-2 in pharyngeal swab tests. Exposures: Patients who died of coronavirus disease 2019. Main Outcomes and Measures: Incidence of SARS-CoV-2 positivity in cardiac tissue as well as CD3 +, CD45 +, and CD68 +cells in the myocardium and gene expression of tumor necrosis growth factor α, interferon γ, chemokine ligand 5, as well as interleukin-6, -8, and -18. Results: Cardiac tissue from 39 consecutive autopsy cases were included. The median (interquartile range) age of patients was 85 (78-89) years, and 23 (59.0%) were women. SARS-CoV-2 could be documented in 24 of 39 patients (61.5%). Viral load above 1000 copies per μg RNA could be documented in 16 of 39 patients (41.0%). A cytokine response panel consisting of 6 proinflammatory genes was increased in those 16 patients compared with 15 patients without any SARS-CoV-2 in the heart. Comparison of 15 patients without cardiac infection with 16 patients with more than 1000 copies revealed no inflammatory cell infiltrates or differences in leukocyte numbers per high power field. Conclusions and Relevance: In this analysis of autopsy cases, viral presence within the myocardium could be documented. While a response to this infection could be reported in cases with higher virus load vs no virus infection, this was not associated with an influx of inflammatory cells. Future investigations should focus on evaluating the long-term consequences of this cardiac involvement. ",
author = "Diana Lindner and Antonia Fitzek and Hanna Brauninger and Ganna Aleshcheva and Caroline Edler and Kira Meissner and Katharina Scherschel and Paulus Kirchhof and Felicitas Escher and Heinz-Peter Schultheiss and Blankenberg, {Stefan S} and Klaus Puschel and Dirk Westermann",
year = "2020",
month = jul,
day = "27",
doi = "10.1001/jamacardio.2020.3551",
language = "English",
journal = "JAMA Cardiology",
issn = "2380-6583",
publisher = "American Medical Association",

}

RIS

TY - JOUR

T1 - Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases

AU - Lindner, Diana

AU - Fitzek, Antonia

AU - Brauninger, Hanna

AU - Aleshcheva, Ganna

AU - Edler, Caroline

AU - Meissner, Kira

AU - Scherschel, Katharina

AU - Kirchhof, Paulus

AU - Escher, Felicitas

AU - Schultheiss, Heinz-Peter

AU - Blankenberg, Stefan S

AU - Puschel, Klaus

AU - Westermann, Dirk

PY - 2020/7/27

Y1 - 2020/7/27

N2 - Importance: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be documented in various tissues, but the frequency of cardiac involvement as well as possible consequences are unknown. Objective: To evaluate the presence of SARS-CoV-2 in the myocardial tissue from autopsy cases and to document a possible cardiac response to that infection. Design, Setting, and Participants: This cohort study used data from consecutive autopsy cases from Germany between April 8 and April 18, 2020. All patients had tested positive for SARS-CoV-2 in pharyngeal swab tests. Exposures: Patients who died of coronavirus disease 2019. Main Outcomes and Measures: Incidence of SARS-CoV-2 positivity in cardiac tissue as well as CD3 +, CD45 +, and CD68 +cells in the myocardium and gene expression of tumor necrosis growth factor α, interferon γ, chemokine ligand 5, as well as interleukin-6, -8, and -18. Results: Cardiac tissue from 39 consecutive autopsy cases were included. The median (interquartile range) age of patients was 85 (78-89) years, and 23 (59.0%) were women. SARS-CoV-2 could be documented in 24 of 39 patients (61.5%). Viral load above 1000 copies per μg RNA could be documented in 16 of 39 patients (41.0%). A cytokine response panel consisting of 6 proinflammatory genes was increased in those 16 patients compared with 15 patients without any SARS-CoV-2 in the heart. Comparison of 15 patients without cardiac infection with 16 patients with more than 1000 copies revealed no inflammatory cell infiltrates or differences in leukocyte numbers per high power field. Conclusions and Relevance: In this analysis of autopsy cases, viral presence within the myocardium could be documented. While a response to this infection could be reported in cases with higher virus load vs no virus infection, this was not associated with an influx of inflammatory cells. Future investigations should focus on evaluating the long-term consequences of this cardiac involvement.

AB - Importance: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be documented in various tissues, but the frequency of cardiac involvement as well as possible consequences are unknown. Objective: To evaluate the presence of SARS-CoV-2 in the myocardial tissue from autopsy cases and to document a possible cardiac response to that infection. Design, Setting, and Participants: This cohort study used data from consecutive autopsy cases from Germany between April 8 and April 18, 2020. All patients had tested positive for SARS-CoV-2 in pharyngeal swab tests. Exposures: Patients who died of coronavirus disease 2019. Main Outcomes and Measures: Incidence of SARS-CoV-2 positivity in cardiac tissue as well as CD3 +, CD45 +, and CD68 +cells in the myocardium and gene expression of tumor necrosis growth factor α, interferon γ, chemokine ligand 5, as well as interleukin-6, -8, and -18. Results: Cardiac tissue from 39 consecutive autopsy cases were included. The median (interquartile range) age of patients was 85 (78-89) years, and 23 (59.0%) were women. SARS-CoV-2 could be documented in 24 of 39 patients (61.5%). Viral load above 1000 copies per μg RNA could be documented in 16 of 39 patients (41.0%). A cytokine response panel consisting of 6 proinflammatory genes was increased in those 16 patients compared with 15 patients without any SARS-CoV-2 in the heart. Comparison of 15 patients without cardiac infection with 16 patients with more than 1000 copies revealed no inflammatory cell infiltrates or differences in leukocyte numbers per high power field. Conclusions and Relevance: In this analysis of autopsy cases, viral presence within the myocardium could be documented. While a response to this infection could be reported in cases with higher virus load vs no virus infection, this was not associated with an influx of inflammatory cells. Future investigations should focus on evaluating the long-term consequences of this cardiac involvement.

UR - http://www.scopus.com/inward/record.url?scp=85089072493&partnerID=8YFLogxK

U2 - 10.1001/jamacardio.2020.3551

DO - 10.1001/jamacardio.2020.3551

M3 - Article

C2 - 32730555

JO - JAMA Cardiology

JF - JAMA Cardiology

SN - 2380-6583

ER -