Assignment of the orphan nuclear receptor Nurr1 by NMR

Research output: Contribution to journalArticle

Authors

  • P Michiels
  • Sara Whittaker
  • M van Dongen

External organisations

  • Solvay Pharmaceuticals Research Laboratories, Hannover, Germany

Abstract

The orphan nuclear receptor Nurr1 has been implicated in a number of conditions including Parkinson's disease and Schizophrenia. As such, it is of interest to study its interactions with other proteins, possibly mediated by small molecules, considering possible use as a drug target. We produced H-2, N-15, C-13 labelled-Nurr1 to generate the backbone amide NH, carbonyl C', C-alpha and C-beta assignments. About 84.0% of residues could be assigned. Most of the 37 missing assignments fall in 3 regions of the protein. Two of these surround a putative ligand-binding region of Nurr1, suggesting that this region of the protein is flexible, despite the ligand-binding pocket being filled with hydrophobic side-chains from residues surrounding the ligand binding pocket.

Details

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalBiomolecular NMR Assignments
Volume4
Issue number1
Early online date19 Mar 2010
Publication statusPublished - 1 Apr 2010

Keywords

  • NR4A nuclear receptors, Orphan nuclear receptor, Parkinson's disease, Nurr1, NMR