Abstract
This study investigated whether changes in the cellular composition of blood during exercise could partly account for observations of exercise-induced changes in lymphocyte oxidative stress markers. Markers of oxidative stress were assessed before and after 60 min of intense treadmill running. Samples were collected from 16 men (means ± SD: age 33 ± 13 yr; body mass index 23.8 ± 2.5 kg/m(2); maximal oxygen uptake 59.7 ± 5.2 ml·kg(-1)·min(-1)). Peripheral blood lymphocytes were assayed for protein carbonyl concentration, and plasma was assessed for lipid peroxides and antioxidant capacity. In a separate study, intracellular thiol concentration was determined in lymphocyte subsets from eight characteristically similar men by flow cytometry, of which T-cell memory populations were further identified on the basis of CD27, CD28, and CD45RA expression. Total lymphocyte protein carbonyls were transiently increased with exercise and returned to baseline within 15 min (P
| Original language | English |
|---|---|
| Pages (from-to) | 206-211 |
| Number of pages | 6 |
| Journal | Journal of Applied Physiology |
| Volume | 111 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jul 2011 |
Keywords
- Adult
- Analysis of Variance
- Antigens
- CD27
- CD28
- CD45
- Biological Markers
- Exercise
- Exercise Test
- Flow Cytometry
- Glutathione
- Humans
- Immunologic Memory
- Immunophenotyping
- Lipid Peroxidation
- Lymphocytes
- Male
- Middle Aged
- Oxidative Stress
- Protein Carbonylation
- Running
- Sulfhydryl Compounds
- T-Lymphocyte Subsets
- Time Factors
- Young Adult