Armadillo-related proteins promote lateral root development in Arabidopsis

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

Armadillo/beta-catenin and related proteins have important functions during animal and Dictyostelium development, regulating cell differentiation, proliferation, and adhesion. Armadillo-repeat-containing proteins also exist in plants, but the majority have unknown roles. The Arabidopsis genes that show greatest sequence homology to Armadillo/beta-catenin are called ARABIDILLO-1 and -2. Here, we demonstrate that ARABIDILLO-1 and -2 promote lateral root development. arabidillo-1/-2 mutants form fewer lateral roots, and ARABIDILLO-1-overexpressing lines produce more lateral roots than wild-type seedlings. ARABIDILLO-yellow fluorescent protein fusions are nuclear. ARABIDILLO proteins contain an F-box motif, and thus may target other proteins for proteasomal degradation. Overexpression of ARABIDILLO-1 protein fragments, including F-box fragments, in wild-type seedlings reduces lateral root formation to the level of the arabidillo-1/-2 mutant. We have shown that plant beta-catenin-related proteins regulate root development. We suggest that ARABIDILLO proteins may target an inhibitor of lateral root development for degradation and propose that Arabidopsis beta-catenin-related proteins define a previously uncharacterized pathway that promotes root branching.

Details

Original languageEnglish
Pages (from-to)1621-6
Number of pages6
JournalNational Academy of Sciences. Proceedings
Volume103
Issue number5
Publication statusPublished - 31 Jan 2006

Keywords

  • Animals, Arabidopsis, Arabidopsis Proteins, Armadillo Domain Proteins, Bacterial Proteins, Cell Adhesion, Cell Differentiation, Cell Nucleus, Cloning, Molecular, DNA Mutational Analysis, Dictyostelium, Luminescent Proteins, Models, Genetic, Mutation, Phenotype, Plant Physiological Phenomena, Plant Proteins, Plant Roots, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, beta Catenin