Architectures of lipid transport systems for the bacterial outer membrane

Research output: Contribution to journalArticlepeer-review

Authors

  • Damian C. Ekiert
  • Gira Bhabha
  • Garrett Greenan
  • Sergey Ovchinnikov
  • Jeffery S. Cox
  • Ronald D. Vale

Colleges, School and Institutes

Abstract

How phospholipids are trafficked between the bacterial inner and outer membranes through the hydrophilic space of the periplasm is not known. We report that members of the mammalian cell entry (MCE) protein family form hexameric assemblies with a central channel capable of mediating lipid transport. The E. coli MCE protein, MlaD, forms a ring associated with an ABC transporter complex in the inner membrane. A soluble lipid-binding protein, MlaC, ferries lipids between MlaD and an outer membrane protein complex. In contrast, EM structures of two other E. coli MCE proteins show that YebT forms an elongated tube consisting of seven stacked MCE rings, and PqiB adopts a syringe-like architecture. Both YebT and PqiB create channels of sufficient length to span the periplasmic space. This work reveals diverse architectures of highly conserved protein-based channels implicated in the transport of lipids between the membranes of bacteria and some eukaryotic organelles.

Details

Original languageEnglish
Pages (from-to)273-285.e17
JournalCell
Volume169
Issue number2
Early online date6 Apr 2017
Publication statusPublished - 6 Apr 2017

Keywords

  • Cell Membrane/chemistry, Crystallography, X-Ray, Escherichia coli/chemistry, Escherichia coli Proteins/chemistry, Membrane Proteins/chemistry, Microscopy, Electron, Models, Molecular, Multiprotein Complexes/chemistry