Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter

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Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter. / Tebbutt, John; Rhodius, Virgil; Webster, Christine; Busby, Stephen.

In: FEMS Microbiology Letters, Vol. 210, No. 1, 01.04.2002, p. 55-60.

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@article{d005c538b15940b7982d7aee94e13c13,
title = "Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter",
abstract = "The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",
keywords = "CRP-dependent promoter, RNA polymerase alpha subunit, cAMP receptor protein, transcription activation",
author = "John Tebbutt and Virgil Rhodius and Christine Webster and Stephen Busby",
year = "2002",
month = apr,
day = "1",
doi = "10.1111/j.1574-6968.2002.tb11159.x",
language = "English",
volume = "210",
pages = "55--60",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter

AU - Tebbutt, John

AU - Rhodius, Virgil

AU - Webster, Christine

AU - Busby, Stephen

PY - 2002/4/1

Y1 - 2002/4/1

N2 - The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - CRP-dependent promoter

KW - RNA polymerase alpha subunit

KW - cAMP receptor protein

KW - transcription activation

UR - http://www.scopus.com/inward/record.url?scp=0037161194&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2002.tb11159.x

DO - 10.1111/j.1574-6968.2002.tb11159.x

M3 - Article

C2 - 12023077

VL - 210

SP - 55

EP - 60

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -