Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter

John Tebbutt; Virgil Rhodius; Christine Webster; Stephen Busby

doi:10.1111/j.1574-6968.2002.tb11159.x

Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter

John Tebbutt, Virgil Rhodius, Christine Webster, Stephen Busby

Biosciences

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	55-60
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	210
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.2002.tb11159.x
Publication status	Published - 1 Apr 2002

Keywords

CRP-dependent promoter
RNA polymerase alpha subunit
cAMP receptor protein
transcription activation

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abstract = "The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",

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T1 - Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter

AU - Tebbutt, John

AU - Rhodius, Virgil

AU - Webster, Christine

AU - Busby, Stephen

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Y1 - 2002/4/1

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AB - The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - CRP-dependent promoter

KW - RNA polymerase alpha subunit

KW - cAMP receptor protein

KW - transcription activation

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Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter

Abstract

Keywords

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