Arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long-term virus accumulation
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Arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long-term virus accumulation. / Miozzi, Laura; Catoni, Marco; Fiorilli, Valentina; Mullineaux, Philip M; Accotto, Gian Paolo; Lanfranco, Luisa.
In: Molecular Plant-Microbe Interactions, Vol. 24, No. 12, 12.2011, p. 1562-72.Research output: Contribution to journal › Article › peer-review
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T1 - Arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long-term virus accumulation
AU - Miozzi, Laura
AU - Catoni, Marco
AU - Fiorilli, Valentina
AU - Mullineaux, Philip M
AU - Accotto, Gian Paolo
AU - Lanfranco, Luisa
PY - 2011/12
Y1 - 2011/12
N2 - Tomato (Solanum lycopersicum) can establish symbiotic interactions with arbuscular mycorrhizal (AM) fungi, and can be infected by several pathogenic viruses. Here, we investigated the impact of mycorrhization by the fungus Glomus mosseae on the Tomato spotted wilt virus (TSWV) infection of tomato plants by transcriptomic and hormones level analyses. In TSWV-infected mycorrhizal plants, the AM fungus root colonization limited virus-induced changes in gene expression in the aerial parts. The virus-responsive upregulated genes, no longer induced in infected mycorrhizal plants, were mainly involved in defense responses and hormone signaling, while the virus-responsive downregulated genes, no longer repressed in mycorrhizal plants, were involved in primary metabolism. The presence of the AM fungus limits, in a salicylic acid-independent manner, the accumulation of abscissic acid observed in response to viral infection. At the time of the molecular analysis, no differences in virus concentration or symptom severity were detected between mycorrhizal and nonmycorrhizal plants. However, in a longer period, increase in virus titer and delay in the appearance of recovery were observed in mycorrhizal plants, thus indicating that the plant's reaction to TSWV infection is attenuated by mycorrhization.
AB - Tomato (Solanum lycopersicum) can establish symbiotic interactions with arbuscular mycorrhizal (AM) fungi, and can be infected by several pathogenic viruses. Here, we investigated the impact of mycorrhization by the fungus Glomus mosseae on the Tomato spotted wilt virus (TSWV) infection of tomato plants by transcriptomic and hormones level analyses. In TSWV-infected mycorrhizal plants, the AM fungus root colonization limited virus-induced changes in gene expression in the aerial parts. The virus-responsive upregulated genes, no longer induced in infected mycorrhizal plants, were mainly involved in defense responses and hormone signaling, while the virus-responsive downregulated genes, no longer repressed in mycorrhizal plants, were involved in primary metabolism. The presence of the AM fungus limits, in a salicylic acid-independent manner, the accumulation of abscissic acid observed in response to viral infection. At the time of the molecular analysis, no differences in virus concentration or symptom severity were detected between mycorrhizal and nonmycorrhizal plants. However, in a longer period, increase in virus titer and delay in the appearance of recovery were observed in mycorrhizal plants, thus indicating that the plant's reaction to TSWV infection is attenuated by mycorrhization.
KW - Abscisic Acid/analysis
KW - Biomass
KW - Cyclopentanes/analysis
KW - Down-Regulation/genetics
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Plant/genetics
KW - Glomeromycota/genetics
KW - Lycopersicon esculentum/genetics
KW - Mycorrhizae/genetics
KW - Oligonucleotide Array Sequence Analysis
KW - Oxylipins/analysis
KW - Phenotype
KW - Plant Diseases/microbiology
KW - Plant Growth Regulators/analysis
KW - Plant Leaves/genetics
KW - Plant Roots/genetics
KW - Plant Shoots/genetics
KW - Salicylic Acid/analysis
KW - Signal Transduction
KW - Symbiosis
KW - Time Factors
KW - Tospovirus/physiology
KW - Transcriptome
KW - Up-Regulation/genetics
U2 - 10.1094/MPMI-05-11-0116
DO - 10.1094/MPMI-05-11-0116
M3 - Article
C2 - 21899386
VL - 24
SP - 1562
EP - 1572
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
SN - 0894-0282
IS - 12
ER -