Arabidopsis PCH2 mediates meiotic chromosome remodeling and maturation of crossovers

Research output: Contribution to journalArticlepeer-review

Standard

Arabidopsis PCH2 mediates meiotic chromosome remodeling and maturation of crossovers. / Lambing, Christophe; Osman, Kim; Nuntasoontorn, Komsun; West, Allan; Higgins, James D; Copenhaver, Gregory P; Yang, Jianhua; Armstrong, Susan J; Mechtler, Karl; Roitinger, Elisabeth; Franklin, F Chris H.

In: PLoS Genetics, Vol. 11, No. 7, e1005372, 16.07.2015.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Bibtex

@article{33bb501c85564bed9e8013eb9689769b,
title = "Arabidopsis PCH2 mediates meiotic chromosome remodeling and maturation of crossovers",
abstract = "Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of the axis occurs during prophase I of meiosis. Structured illumination microscopy (SIM) has revealed dynamic changes in the chromosome axis in Arabidopsis thaliana and Brassica oleracea. We show that the axis associated protein ASY1 is depleted during zygotene concomitant with synaptonemal complex (SC) formation. Study of an Atpch2 mutant demonstrates this requires the conserved AAA+ ATPase, PCH2, which localizes to the sites of axis remodelling. Loss of PCH2 leads to a failure to deplete ASY1 from the axes and compromizes SC polymerisation. Immunolocalization of recombination proteins in Atpch2 indicates that recombination initiation and CO designation during early prophase I occur normally. Evidence suggests that CO interference is initially functional in the mutant but there is a defect in CO maturation following designation. This leads to a reduction in COs and a failure to form COs between some homologous chromosome pairs leading to univalent chromosomes at metaphase I. Genetic analysis reveals that CO distribution is also affected in some chromosome regions. Together these data indicate that the axis remodelling defect in Atpch2 disrupts normal patterned formation of COs.",
keywords = "Arabidopsis thaliana, Recombinant proteins, Prophase, Chromosomes, crossover interference, DNA recombination, Saccharomyces cerevisiae, Genetic interference",
author = "Christophe Lambing and Kim Osman and Komsun Nuntasoontorn and Allan West and Higgins, {James D} and Copenhaver, {Gregory P} and Jianhua Yang and Armstrong, {Susan J} and Karl Mechtler and Elisabeth Roitinger and Franklin, {F Chris H}",
year = "2015",
month = jul,
day = "16",
doi = "10.1371/journal.pgen.1005372",
language = "English",
volume = "11",
journal = "PLoS Genetics",
issn = "1553-7390",
publisher = "Public Library of Science (PLOS)",
number = "7",

}

RIS

TY - JOUR

T1 - Arabidopsis PCH2 mediates meiotic chromosome remodeling and maturation of crossovers

AU - Lambing, Christophe

AU - Osman, Kim

AU - Nuntasoontorn, Komsun

AU - West, Allan

AU - Higgins, James D

AU - Copenhaver, Gregory P

AU - Yang, Jianhua

AU - Armstrong, Susan J

AU - Mechtler, Karl

AU - Roitinger, Elisabeth

AU - Franklin, F Chris H

PY - 2015/7/16

Y1 - 2015/7/16

N2 - Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of the axis occurs during prophase I of meiosis. Structured illumination microscopy (SIM) has revealed dynamic changes in the chromosome axis in Arabidopsis thaliana and Brassica oleracea. We show that the axis associated protein ASY1 is depleted during zygotene concomitant with synaptonemal complex (SC) formation. Study of an Atpch2 mutant demonstrates this requires the conserved AAA+ ATPase, PCH2, which localizes to the sites of axis remodelling. Loss of PCH2 leads to a failure to deplete ASY1 from the axes and compromizes SC polymerisation. Immunolocalization of recombination proteins in Atpch2 indicates that recombination initiation and CO designation during early prophase I occur normally. Evidence suggests that CO interference is initially functional in the mutant but there is a defect in CO maturation following designation. This leads to a reduction in COs and a failure to form COs between some homologous chromosome pairs leading to univalent chromosomes at metaphase I. Genetic analysis reveals that CO distribution is also affected in some chromosome regions. Together these data indicate that the axis remodelling defect in Atpch2 disrupts normal patterned formation of COs.

AB - Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of the axis occurs during prophase I of meiosis. Structured illumination microscopy (SIM) has revealed dynamic changes in the chromosome axis in Arabidopsis thaliana and Brassica oleracea. We show that the axis associated protein ASY1 is depleted during zygotene concomitant with synaptonemal complex (SC) formation. Study of an Atpch2 mutant demonstrates this requires the conserved AAA+ ATPase, PCH2, which localizes to the sites of axis remodelling. Loss of PCH2 leads to a failure to deplete ASY1 from the axes and compromizes SC polymerisation. Immunolocalization of recombination proteins in Atpch2 indicates that recombination initiation and CO designation during early prophase I occur normally. Evidence suggests that CO interference is initially functional in the mutant but there is a defect in CO maturation following designation. This leads to a reduction in COs and a failure to form COs between some homologous chromosome pairs leading to univalent chromosomes at metaphase I. Genetic analysis reveals that CO distribution is also affected in some chromosome regions. Together these data indicate that the axis remodelling defect in Atpch2 disrupts normal patterned formation of COs.

KW - Arabidopsis thaliana

KW - Recombinant proteins

KW - Prophase

KW - Chromosomes

KW - crossover interference

KW - DNA recombination

KW - Saccharomyces cerevisiae

KW - Genetic interference

U2 - 10.1371/journal.pgen.1005372

DO - 10.1371/journal.pgen.1005372

M3 - Article

C2 - 26182244

VL - 11

JO - PLoS Genetics

JF - PLoS Genetics

SN - 1553-7390

IS - 7

M1 - e1005372

ER -