Application of ex situ dynamic nuclear polarization in studying small molecules
Research output: Contribution to journal › Article › peer-review
Dynamic nuclear polarization (DNP) has become an attractive technique to boost the sensitivity of NMR experiments. In the case of ex situ polarizations two-dimensional (2D) spectra are limited by the short lifetime of the polarization after dissolution and sample transfer to a high field NMR magnet. This limitation can be overcome by various approaches. Here we show how the use of 13C-labelled acetyl tags can help to obtain 2D-HMQC spectra for many small molecules, owing to a nuclear Overhauser enhancement between 13C spins originating from the long-lived carbonyl carbon, which extends the lifetimes of other 13C spins with shorter longitudinal relaxation times. We also show an alternative approach of using an optimized polarization matrix.
|Number of pages||4|
|Journal||Physical Chemistry Chemical Physics|
|Early online date||10 May 2010|
|Publication status||Published - 14 Jun 2010|
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