Application of ex situ dynamic nuclear polarization in studying small molecules

Research output: Contribution to journalArticle

Abstract

Dynamic nuclear polarization (DNP) has become an attractive technique to boost the sensitivity of NMR experiments. In the case of ex situ polarizations two-dimensional (2D) spectra are limited by the short lifetime of the polarization after dissolution and sample transfer to a high field NMR magnet. This limitation can be overcome by various approaches. Here we show how the use of 13C-labelled acetyl tags can help to obtain 2D-HMQC spectra for many small molecules, owing to a nuclear Overhauser enhancement between 13C spins originating from the long-lived carbonyl carbon, which extends the lifetimes of other 13C spins with shorter longitudinal relaxation times. We also show an alternative approach of using an optimized polarization matrix.

Details

Original languageEnglish
Pages (from-to)5868-5871
Number of pages4
JournalPhysical Chemistry Chemical Physics
Volume12
Issue number22
Early online date10 May 2010
Publication statusPublished - 14 Jun 2010

Keywords

  • NR4A nuclear receptors, Orphan nuclear receptor, Parkinson's disease, Nurr1, NMR