Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

Emma Jennings; David Lecky; Masahiro Ono; David Bending

doi:10.1016/j.xpro.2020.100284

Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

Emma Jennings, David Lecky, Masahiro Ono, David Bending

Immunology and Immunotherapy

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Abstract

This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining.

For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020.

Original language	English
Article number	100284
Journal	STAR Protocols
Volume	2
Issue number	1
Early online date	21 Jan 2021
DOIs	https://doi.org/10.1016/j.xpro.2020.100284
Publication status	Published - 19 Mar 2021

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10.1016/j.xpro.2020.100284Licence: Creative Commons: Attribution (CC BY)

JenningsEK2021ApplicationFinal published version, 1.82 MBLicence: Creative Commons: Attribution (CC BY)

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Negative feedback control of T cells in tolerance and cancer - from pathways to biomarkers
Bending, D.
Medical Research Council
1/02/21 → 31/01/26
Project: Research Councils
How do T Cell Receptor signalling dynamics regulate interleukin-10-producing T-cell differentiation?
Bending, D.
THE WELLCOME TRUST
5/11/18 → 5/05/21
Project: Research

Cite this

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AU - Bending, David

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Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

Abstract

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Projects

Negative feedback control of T cells in tolerance and cancer - from pathways to biomarkers

How do T Cell Receptor signalling dynamics regulate interleukin-10-producing T-cell differentiation?

Cite this