Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting

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Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting. / Snel, Alexander N; Kelder, Angèle; Scholten, Willemijn J; Khan, Naeem; Metzner, Marlen; Irno-Consalvo, Maria; Sugita, Mayumi; de Jong, Anja; Oude Alink, Sjoerd; Eidhof, Harrie; Wilhelm, Miriam; Feuring-Buske, Michaela; Slomp, Jennichjen; van der Velden, Vincent H J; Sonneveld, Edwin; Guzman, Monica; Roboz, Gail J; Buccisano, Francesco; Vyas, Paresh; Freeman, Sylvie; Bachas, Costa; Ossenkoppele, Gert J; Schuurhuis, Gerrit J; Cloos, Jacqueline.

In: British Journal of Haematology, 02.04.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Snel, AN, Kelder, A, Scholten, WJ, Khan, N, Metzner, M, Irno-Consalvo, M, Sugita, M, de Jong, A, Oude Alink, S, Eidhof, H, Wilhelm, M, Feuring-Buske, M, Slomp, J, van der Velden, VHJ, Sonneveld, E, Guzman, M, Roboz, GJ, Buccisano, F, Vyas, P, Freeman, S, Bachas, C, Ossenkoppele, GJ, Schuurhuis, GJ & Cloos, J 2020, 'Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting', British Journal of Haematology. https://doi.org/10.1111/bjh.16594

APA

Snel, A. N., Kelder, A., Scholten, W. J., Khan, N., Metzner, M., Irno-Consalvo, M., Sugita, M., de Jong, A., Oude Alink, S., Eidhof, H., Wilhelm, M., Feuring-Buske, M., Slomp, J., van der Velden, V. H. J., Sonneveld, E., Guzman, M., Roboz, G. J., Buccisano, F., Vyas, P., ... Cloos, J. (2020). Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting. British Journal of Haematology. https://doi.org/10.1111/bjh.16594

Vancouver

Author

Snel, Alexander N ; Kelder, Angèle ; Scholten, Willemijn J ; Khan, Naeem ; Metzner, Marlen ; Irno-Consalvo, Maria ; Sugita, Mayumi ; de Jong, Anja ; Oude Alink, Sjoerd ; Eidhof, Harrie ; Wilhelm, Miriam ; Feuring-Buske, Michaela ; Slomp, Jennichjen ; van der Velden, Vincent H J ; Sonneveld, Edwin ; Guzman, Monica ; Roboz, Gail J ; Buccisano, Francesco ; Vyas, Paresh ; Freeman, Sylvie ; Bachas, Costa ; Ossenkoppele, Gert J ; Schuurhuis, Gerrit J ; Cloos, Jacqueline. / Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting. In: British Journal of Haematology. 2020.

Bibtex

@article{2a050fb8f4b84563b6ee5838b520d105,
title = "Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting",
abstract = "Leukaemic stem cells (LSC) have been experimentally defined as the leukaemia-propagating population and are thought to be the cellular reservoir of relapse in acute myeloid leukaemia (AML). Therefore, LSC measurements are warranted to facilitate accurate risk stratification. Previously, we published the composition of a one-tube flow cytometric assay, characterised by the presence of 13 important membrane markers for LSC detection. Here we present the validation experiments of the assay in several large AML research centres, both in Europe and the United States. Variability within instruments and sample processing showed high correlations between different instruments (Rpearson > 0·91, P < 0·001). Multi-centre testing introduced variation in reported LSC percentages but was found to be below the clinical relevant threshold. Clear gating protocols resulted in all laboratories being able to perform LSC assessment of the validation set. Participating centres were nearly unanimously able to distinguish LSChigh (>0·03% LSC) from LSClow (<0·03% LSC) despite inter-laboratory variation in reported LSC percentages. This study proves that the LSC assay is highly reproducible. These results together with the high prognostic impact of LSC load at diagnosis in AML patients render the one-tube LSC assessment a good marker for future risk classification.",
author = "Snel, {Alexander N} and Ang{\`e}le Kelder and Scholten, {Willemijn J} and Naeem Khan and Marlen Metzner and Maria Irno-Consalvo and Mayumi Sugita and {de Jong}, Anja and {Oude Alink}, Sjoerd and Harrie Eidhof and Miriam Wilhelm and Michaela Feuring-Buske and Jennichjen Slomp and {van der Velden}, {Vincent H J} and Edwin Sonneveld and Monica Guzman and Roboz, {Gail J} and Francesco Buccisano and Paresh Vyas and Sylvie Freeman and Costa Bachas and Ossenkoppele, {Gert J} and Schuurhuis, {Gerrit J} and Jacqueline Cloos",
note = "{\textcopyright} 2020 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.",
year = "2020",
month = apr,
day = "2",
doi = "10.1111/bjh.16594",
language = "English",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "Wiley",

}

RIS

TY - JOUR

T1 - Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting

AU - Snel, Alexander N

AU - Kelder, Angèle

AU - Scholten, Willemijn J

AU - Khan, Naeem

AU - Metzner, Marlen

AU - Irno-Consalvo, Maria

AU - Sugita, Mayumi

AU - de Jong, Anja

AU - Oude Alink, Sjoerd

AU - Eidhof, Harrie

AU - Wilhelm, Miriam

AU - Feuring-Buske, Michaela

AU - Slomp, Jennichjen

AU - van der Velden, Vincent H J

AU - Sonneveld, Edwin

AU - Guzman, Monica

AU - Roboz, Gail J

AU - Buccisano, Francesco

AU - Vyas, Paresh

AU - Freeman, Sylvie

AU - Bachas, Costa

AU - Ossenkoppele, Gert J

AU - Schuurhuis, Gerrit J

AU - Cloos, Jacqueline

N1 - © 2020 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.

PY - 2020/4/2

Y1 - 2020/4/2

N2 - Leukaemic stem cells (LSC) have been experimentally defined as the leukaemia-propagating population and are thought to be the cellular reservoir of relapse in acute myeloid leukaemia (AML). Therefore, LSC measurements are warranted to facilitate accurate risk stratification. Previously, we published the composition of a one-tube flow cytometric assay, characterised by the presence of 13 important membrane markers for LSC detection. Here we present the validation experiments of the assay in several large AML research centres, both in Europe and the United States. Variability within instruments and sample processing showed high correlations between different instruments (Rpearson > 0·91, P < 0·001). Multi-centre testing introduced variation in reported LSC percentages but was found to be below the clinical relevant threshold. Clear gating protocols resulted in all laboratories being able to perform LSC assessment of the validation set. Participating centres were nearly unanimously able to distinguish LSChigh (>0·03% LSC) from LSClow (<0·03% LSC) despite inter-laboratory variation in reported LSC percentages. This study proves that the LSC assay is highly reproducible. These results together with the high prognostic impact of LSC load at diagnosis in AML patients render the one-tube LSC assessment a good marker for future risk classification.

AB - Leukaemic stem cells (LSC) have been experimentally defined as the leukaemia-propagating population and are thought to be the cellular reservoir of relapse in acute myeloid leukaemia (AML). Therefore, LSC measurements are warranted to facilitate accurate risk stratification. Previously, we published the composition of a one-tube flow cytometric assay, characterised by the presence of 13 important membrane markers for LSC detection. Here we present the validation experiments of the assay in several large AML research centres, both in Europe and the United States. Variability within instruments and sample processing showed high correlations between different instruments (Rpearson > 0·91, P < 0·001). Multi-centre testing introduced variation in reported LSC percentages but was found to be below the clinical relevant threshold. Clear gating protocols resulted in all laboratories being able to perform LSC assessment of the validation set. Participating centres were nearly unanimously able to distinguish LSChigh (>0·03% LSC) from LSClow (<0·03% LSC) despite inter-laboratory variation in reported LSC percentages. This study proves that the LSC assay is highly reproducible. These results together with the high prognostic impact of LSC load at diagnosis in AML patients render the one-tube LSC assessment a good marker for future risk classification.

U2 - 10.1111/bjh.16594

DO - 10.1111/bjh.16594

M3 - Article

C2 - 32239670

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

ER -