Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting
Research output: Contribution to journal › Article › peer-review
- Universitatsklinikum, Hematology Department, Freiburg, Germany.
- University of Rome Tor Vergata
- Weill Cornell Medical College
- Dutch Childhood Oncology Group
- Department of Immunology, Erasmus Medical Center, University Medical Center, Rotterdam, the Netherlands.
- University Hospitals Birmingham NHS Foundation Trust
- University Hospital of Ulm
- University of Oxford
- Oxford University Hospitals NHS Foundation Trust
Leukaemic stem cells (LSC) have been experimentally defined as the leukaemia-propagating population and are thought to be the cellular reservoir of relapse in acute myeloid leukaemia (AML). Therefore, LSC measurements are warranted to facilitate accurate risk stratification. Previously, we published the composition of a one-tube flow cytometric assay, characterised by the presence of 13 important membrane markers for LSC detection. Here we present the validation experiments of the assay in several large AML research centres, both in Europe and the United States. Variability within instruments and sample processing showed high correlations between different instruments (Rpearson > 0·91, P < 0·001). Multi-centre testing introduced variation in reported LSC percentages but was found to be below the clinical relevant threshold. Clear gating protocols resulted in all laboratories being able to perform LSC assessment of the validation set. Participating centres were nearly unanimously able to distinguish LSChigh (>0·03% LSC) from LSClow (<0·03% LSC) despite inter-laboratory variation in reported LSC percentages. This study proves that the LSC assay is highly reproducible. These results together with the high prognostic impact of LSC load at diagnosis in AML patients render the one-tube LSC assessment a good marker for future risk classification.
|Journal||British Journal of Haematology|
|Early online date||2 Apr 2020|
|Publication status||E-pub ahead of print - 2 Apr 2020|