Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces

Leigh  Townsend; Richard Williams; Olachi Anuforom; Matthew Berwick; Fenella Halstead; Erik Hughes; Artemis Stamboulis; Beryl Oppenheim; Julie E. Gough; Liam Grover; Robert Scott; Mark Webber; Anna Peacock; Antonio Belli; Ann Logan; Felicity De Cogan

doi:10.1098/rsif.2016.0657

Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces

Leigh Townsend, Richard Williams, Olachi Anuforom, Matthew Berwick, Fenella Halstead, Erik Hughes, Artemis Stamboulis, Beryl Oppenheim, Julie E. Gough, Liam Grover, Robert Scott, Mark Webber, Anna Peacock, Antonio Belli, Ann Logan, Felicity De Cogan

Research output: Contribution to journal › Article › peer-review

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Abstract

The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material–tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.

Original language	English
Article number	20160657
Number of pages	12
Journal	Journal of The Royal Society Interface
Volume	14
Issue number	126
Early online date	1 Jan 2017
DOIs	https://doi.org/10.1098/rsif.2016.0657
Publication status	Published - 31 Jan 2017

Bibliographical note

Keywords

Animals
Antimicrobial Cationic Peptides/chemistry
Cell Line
Coated Materials, Biocompatible/chemistry
Durapatite/chemistry
Gram-Negative Bacteria/growth & development
Gram-Positive Bacteria/growth & development
Materials Testing
Mice
Osteoblasts/cytology
Static Electricity

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10.1098/rsif.2016.0657Licence: None: All rights reserved

Townsend_et_al_Antimicrobial_Peptide_Coatings_Journal_Royal_Society_Interface_2017
Checked for eligibility: 28/03/17 Published as: Townsend, Leigh, et al. "Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces." Journal of The Royal Society Interface (2017)
Accepted author manuscript, 957 KBLicence: None: All rights reserved

https://royalsocietypublishing.org/doi/10.1098/rsif.2016.0657Licence: None: All rights reserved

Development of a Small Molecule Combinatorial Treatment for RGC Survival and Axon Regeneration to Restore Sight After Optic Neuropathy
Logan, A. & Morgan-Warren, P.
Medical Research Council
1/09/12 → 31/08/15
Project: Research Councils

Cite this

Townsend, L., Williams, R., Anuforom, O., Berwick, M., Halstead, F., Hughes, E., Stamboulis, A., Oppenheim, B., Gough, J. E., Grover, L., Scott, R., Webber, M., Peacock, A., Belli, A., Logan, A., & De Cogan, F. (2017). Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces. Journal of The Royal Society Interface, 14(126), Article 20160657. Advance online publication. https://doi.org/10.1098/rsif.2016.0657

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title = "Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces",

abstract = "The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material–tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.",

keywords = "Animals, Antimicrobial Cationic Peptides/chemistry, Cell Line, Coated Materials, Biocompatible/chemistry, Durapatite/chemistry, Gram-Negative Bacteria/growth & development, Gram-Positive Bacteria/growth & development, Materials Testing, Mice, Osteoblasts/cytology, Static Electricity",

author = "Leigh Townsend and Richard Williams and Olachi Anuforom and Matthew Berwick and Fenella Halstead and Erik Hughes and Artemis Stamboulis and Beryl Oppenheim and Gough, {Julie E.} and Liam Grover and Robert Scott and Mark Webber and Anna Peacock and Antonio Belli and Ann Logan and {De Cogan}, Felicity",

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Townsend, L, Williams, R, Anuforom, O, Berwick, M, Halstead, F, Hughes, E, Stamboulis, A, Oppenheim, B, Gough, JE, Grover, L, Scott, R, Webber, M, Peacock, A , Belli, A, Logan, A & De Cogan, F 2017, 'Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces', Journal of The Royal Society Interface, vol. 14, no. 126, 20160657. https://doi.org/10.1098/rsif.2016.0657

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T1 - Antimicrobial peptide coatings for hydroxyapatite

T2 - electrostatic and covalent attachment of antimicrobial peptides to surfaces

AU - Townsend, Leigh

AU - Williams, Richard

AU - Anuforom, Olachi

AU - Berwick, Matthew

AU - Halstead, Fenella

AU - Hughes, Erik

AU - Stamboulis, Artemis

AU - Oppenheim, Beryl

AU - Gough, Julie E.

AU - Grover, Liam

AU - Scott, Robert

AU - Webber, Mark

AU - Peacock, Anna

AU - Belli, Antonio

AU - Logan, Ann

AU - De Cogan, Felicity

PY - 2017/1/31

Y1 - 2017/1/31

N2 - The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material–tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.

AB - The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material–tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.

KW - Animals

KW - Antimicrobial Cationic Peptides/chemistry

KW - Cell Line

KW - Coated Materials, Biocompatible/chemistry

KW - Durapatite/chemistry

KW - Gram-Negative Bacteria/growth & development

KW - Gram-Positive Bacteria/growth & development

KW - Materials Testing

KW - Mice

KW - Osteoblasts/cytology

KW - Static Electricity

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310730/

UR - https://europepmc.org/article/MED/28077764

U2 - 10.1098/rsif.2016.0657

DO - 10.1098/rsif.2016.0657

M3 - Article

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SN - 1742-5689

VL - 14

JO - Journal of The Royal Society Interface

JF - Journal of The Royal Society Interface

IS - 126

M1 - 20160657

ER -

Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces

Abstract

Bibliographical note

Keywords

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Projects

Development of a Small Molecule Combinatorial Treatment for RGC Survival and Axon Regeneration to Restore Sight After Optic Neuropathy

Cite this