Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces

Research output: Contribution to journalArticlepeer-review

Authors

External organisations

  • University of Birmingham Microbiome Treatment Centre, University of Birmingham, Birmingham, United Kingdom; Department of Gastroenterology, University Hospital Birmingham, Birmingham, United Kingdom. Electronic address: m.n.quraishi@bham.ac.uk.
  • School of Chemical Engineering
  • NIHR Surgical Reconstruction and Microbiology Research Centre
  • Queen Elizabeth Hospital Birmingham, Queen Elizabeth Medical Centre
  • Institute for Inflammation and Ageing
  • School of Chemistry
  • Departments of Cardiology, Sandwell and West Birmingham Hospitals NHS Trust and University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
  • School of Metallurgy and Materials
  • Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UK.
  • ROYAL CENTRE FOR DEFENCE MEDICINE

Abstract

The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material-tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.

Bibliographic note

© 2017 The Author(s).

Details

Original languageEnglish
JournalJournal of The Royal Society Interface
Volume14
Issue number126
Publication statusPublished - Jan 2017

Keywords

  • Animals, Antimicrobial Cationic Peptides/chemistry, Cell Line, Coated Materials, Biocompatible/chemistry, Durapatite/chemistry, Gram-Negative Bacteria/growth & development, Gram-Positive Bacteria/growth & development, Materials Testing, Mice, Osteoblasts/cytology, Static Electricity