Anthracene-modified oligonucleotides as fluorescent DNA mismatch sensors: discrimination between various base-pair mismatches

Jean-Louis Duprey; DM Bassani; Eva Hyde; Christian Ludwig; A Rodger; JS Vyle; John Wilkie; ZY Zhao; James H R Tucker

doi:10.1080/10610278.2010.523117

Anthracene-modified oligonucleotides as fluorescent DNA mismatch sensors: discrimination between various base-pair mismatches

Jean-Louis Duprey, DM Bassani, Eva Hyde, Christian Ludwig, A Rodger, JS Vyle, John Wilkie, ZY Zhao, James H R Tucker

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A fluorescent anthracene-tagged DNA probe has been shown to respond to various DNA sequences by changes to its emission signal upon duplex formation. The fluorescence response for duplexes containing a single mismatch near the anthracene site has been found to be very sensitive to its composition, with the emission signal increasing for a CA mismatch and decreasing for CT and CC mismatches.

Original language	English
Pages (from-to)	273-277
Number of pages	5
Journal	Supramolecular Chemistry
Volume	23
Issue number	3-4
Early online date	13 Apr 2011
DOIs	https://doi.org/10.1080/10610278.2010.523117
Publication status	Published - 2011

Keywords

sensor
fluorescence
anthracene
SNP
DNA

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10.1080/10610278.2010.523117Licence: None: All rights reserved

Functional DNA-based assemblies
Tucker, J.
Engineering & Physical Science Research Council
16/03/09 → 31/03/14
Project: Research Councils

Cite this

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title = "Anthracene-modified oligonucleotides as fluorescent DNA mismatch sensors: discrimination between various base-pair mismatches",

abstract = "A fluorescent anthracene-tagged DNA probe has been shown to respond to various DNA sequences by changes to its emission signal upon duplex formation. The fluorescence response for duplexes containing a single mismatch near the anthracene site has been found to be very sensitive to its composition, with the emission signal increasing for a CA mismatch and decreasing for CT and CC mismatches.",

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author = "Jean-Louis Duprey and DM Bassani and Eva Hyde and Christian Ludwig and A Rodger and JS Vyle and John Wilkie and ZY Zhao and Tucker, {James H R}",

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T2 - discrimination between various base-pair mismatches

AU - Duprey, Jean-Louis

AU - Bassani, DM

AU - Hyde, Eva

AU - Ludwig, Christian

AU - Rodger, A

AU - Vyle, JS

AU - Wilkie, John

AU - Zhao, ZY

AU - Tucker, James H R

PY - 2011

Y1 - 2011

N2 - A fluorescent anthracene-tagged DNA probe has been shown to respond to various DNA sequences by changes to its emission signal upon duplex formation. The fluorescence response for duplexes containing a single mismatch near the anthracene site has been found to be very sensitive to its composition, with the emission signal increasing for a CA mismatch and decreasing for CT and CC mismatches.

AB - A fluorescent anthracene-tagged DNA probe has been shown to respond to various DNA sequences by changes to its emission signal upon duplex formation. The fluorescence response for duplexes containing a single mismatch near the anthracene site has been found to be very sensitive to its composition, with the emission signal increasing for a CA mismatch and decreasing for CT and CC mismatches.

KW - sensor

KW - fluorescence

KW - anthracene

KW - SNP

KW - DNA

U2 - 10.1080/10610278.2010.523117

DO - 10.1080/10610278.2010.523117

M3 - Article

VL - 23

SP - 273

EP - 277

JO - Supramolecular Chemistry

JF - Supramolecular Chemistry

IS - 3-4

ER -

Anthracene-modified oligonucleotides as fluorescent DNA mismatch sensors: discrimination between various base-pair mismatches

Abstract

Keywords

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Projects

Functional DNA-based assemblies

Cite this