Analyzing sister chromatid cohesion in mammalian cells

Katherine M. Feeney; Laura McFarlane-Majeed; Joanna L. Parish

doi:10.1007/978-1-4939-0888-2_32

Analyzing sister chromatid cohesion in mammalian cells

Katherine M. Feeney, Laura McFarlane-Majeed, Joanna L. Parish

Cancer and Genomic Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The metaphase chromosome spread technique and subsequent analysis of sister chromatid cohesion is used for (clinical) diagnosis of genetic abnormalities that can cause aberrant sister chromatid cohesion. In addition, the technique can be used to assess the contribution of novel genes to the cohesion establishment and maintenance pathways. Cells are swelled in a hypotonic solution and fixed in Carnoy’s solution. Samples are then dropped onto glass slides, and the spread chromosomes are stained and visualized by microscopy. Defects in sister chromatid cohesion can be easily assessed using this method, examples of which are given.

Original language	English
Title of host publication	Cell Cycle Control
Subtitle of host publication	Mechanisms and Protocols
Editors	Eishi Noguchi, Mariana C. Gadaleta
Place of Publication	Springer New York
Publisher	Humana Press
Pages	563-569
Number of pages	7
Edition	2nd
ISBN (Electronic)	978-1493908882
ISBN (Print)	978-1493908875
DOIs	https://doi.org/10.1007/978-1-4939-0888-2_32
Publication status	Published - 1 Jan 2014

Publication series

Name	Methods in Molecular Biology
Volume	1170
ISSN (Print)	1064-3745

Keywords

Chromosomes
Cohesin
Cohesion
Metaphase
Sister chromatids

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-0888-2_32

The Functional Role of ChIR1 in Genome Integrity and Papillomavirus Persistence
Parish, J.
The Royal Society
1/02/13 → 31/01/16
Project: Research Councils

Cite this

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Analyzing sister chromatid cohesion in mammalian cells. / Feeney, Katherine M.; McFarlane-Majeed, Laura; Parish, Joanna L.
Cell Cycle Control: Mechanisms and Protocols. ed. / Eishi Noguchi; Mariana C. Gadaleta. 2nd. ed. Springer New York: Humana Press, 2014. p. 563-569 (Methods in Molecular Biology; Vol. 1170).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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Analyzing sister chromatid cohesion in mammalian cells

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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Projects

The Functional Role of ChIR1 in Genome Integrity and Papillomavirus Persistence

Cite this