Analyzing sister chromatid cohesion in mammalian cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Authors

Colleges, School and Institutes

Abstract

The metaphase chromosome spread technique and subsequent analysis of sister chromatid cohesion is used for (clinical) diagnosis of genetic abnormalities that can cause aberrant sister chromatid cohesion. In addition, the technique can be used to assess the contribution of novel genes to the cohesion establishment and maintenance pathways. Cells are swelled in a hypotonic solution and fixed in Carnoy’s solution. Samples are then dropped onto glass slides, and the spread chromosomes are stained and visualized by microscopy. Defects in sister chromatid cohesion can be easily assessed using this method, examples of which are given.

Details

Original languageEnglish
Title of host publicationCell Cycle Control
Subtitle of host publicationMechanisms and Protocols
EditorsEishi Noguchi, Mariana C. Gadaleta
Publication statusPublished - 1 Jan 2014

Publication series

NameMethods in Molecular Biology
Volume1170
ISSN (Print)1064-3745

Keywords

  • Chromosomes, Cohesin, Cohesion, Metaphase, Sister chromatids

ASJC Scopus subject areas