Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression

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Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression. / Fielding, CA; Siebert, S; Rowe, Martin; Brennan, P.

In: FEBS Letters, Vol. 570, 01.07.2004, p. 138-142.

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@article{2453aebff1554154af60a6b4b40819bc,
title = "Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression",
abstract = "Tumour necrosis factor receptor 1 (TNFR1) plays a critical role in host defence and inflammation. We have identified a membrane proximal region (aa 218-324) of TNFR1 that restricts surface expression. This was prompted by comparing the dominant-negative properties of a C-terminal truncation of TNFR1 with a point mutant that prevents signalling. C-terminal truncation (aa 218-426) generates a better dominant-negative TNFR1 mutant than inactivation of the death domain by point mutation. The increased dominant-negative activity correlates with increased cell surface expression. The membrane proximal region is the most important region of the receptor for restricting expression.",
author = "CA Fielding and S Siebert and Martin Rowe and P Brennan",
year = "2004",
month = jul,
day = "1",
doi = "10.1016/j.febslet.2004.06.035",
language = "English",
volume = "570",
pages = "138--142",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression

AU - Fielding, CA

AU - Siebert, S

AU - Rowe, Martin

AU - Brennan, P

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Tumour necrosis factor receptor 1 (TNFR1) plays a critical role in host defence and inflammation. We have identified a membrane proximal region (aa 218-324) of TNFR1 that restricts surface expression. This was prompted by comparing the dominant-negative properties of a C-terminal truncation of TNFR1 with a point mutant that prevents signalling. C-terminal truncation (aa 218-426) generates a better dominant-negative TNFR1 mutant than inactivation of the death domain by point mutation. The increased dominant-negative activity correlates with increased cell surface expression. The membrane proximal region is the most important region of the receptor for restricting expression.

AB - Tumour necrosis factor receptor 1 (TNFR1) plays a critical role in host defence and inflammation. We have identified a membrane proximal region (aa 218-324) of TNFR1 that restricts surface expression. This was prompted by comparing the dominant-negative properties of a C-terminal truncation of TNFR1 with a point mutant that prevents signalling. C-terminal truncation (aa 218-426) generates a better dominant-negative TNFR1 mutant than inactivation of the death domain by point mutation. The increased dominant-negative activity correlates with increased cell surface expression. The membrane proximal region is the most important region of the receptor for restricting expression.

UR - http://www.scopus.com/inward/record.url?scp=3142566343&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2004.06.035

DO - 10.1016/j.febslet.2004.06.035

M3 - Article

C2 - 15251454

VL - 570

SP - 138

EP - 142

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

ER -