An introduction to the methodology of expansion microscopy

Research output: Contribution to journalArticlepeer-review

Colleges, School and Institutes

Abstract

Expansion microscopy is a novel, fluorescence imaging technique, which allows three-dimensional nanoscale imaging of specimens on a conventional fluorescence microscope. This is achieved through an innovative sample treatment, which culminates in approximately 4.5-fold expansion of specimens in each dimension. This allows 70 nm lateral and 200 nm axial resolution. To further develop application of the technique, there has been considerable focus on improving the methodology by i) extending the efficacy of labelling, ii) enabling multi-colour labelling of different biomolecules simultaneously, iii) further improving resolving power through alterations to sample preparation and iv) by combination of expansion microscopy with other well-established super resolution techniques. This review will highlight some of these recent advances and suggest ways that the technique could be developed further in the future.

Details

Original languageEnglish
Article number105764
Number of pages7
JournalThe International Journal of Biochemistry & Cell Biology
Volume124
Early online date12 May 2020
Publication statusPublished - Jul 2020

Keywords

  • Expansion microscopy, Super resolution, Imaging