An in vitro mucosal model for prediction of the bioadhesion of alginate solutions to the oesophagus

Research output: Contribution to journalArticle

Authors

  • D. Banning
  • P.W. Dettmar
  • F.C. Hampson
  • I.G. Jolliffe
  • D.Q.M. Craig

Colleges, School and Institutes

External organisations

  • Queen's University Belfast
  • Reckitt Benckiser
  • LONDON UNIVERSITY
  • Aston University

Abstract

This paper discusses the development of an in vitro model utilised to assess the adhesion of alginate solutions to porcine oesophageal tissue. The methodology involved the construction of retention apparatus onto which sections of tissue were mounted. Fluorescently labelled alginate solutions of known rheological profile were dispensed onto the tissue at a concentration of 2% w/v. A washing solution was applied at a specified rate to mimic saliva flow and the eluted material collected. Fluorimetric analysis allowed dose retention to be assessed as a function of time. The effect of the nature of the washing solution and the choice of alginate were investigated. It was found that after 30 min up to 20% of the applied alginate dose remained associated with the tissue, regardless of the alginate selected from the range examined. The nature of the washing medium did not have a significant effect on retention, irrespective of the inherent mucin concentration. Overall this study indicated that the technique presented offers a viable means of studying bioadhesion of liquids and also demonstrates that alginate solutions may have an application as bioadhesive agents for localisation within the oesophagus.

Details

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalInternational Journal of Pharmaceutics
Volume238
Issue number1-2
Publication statusPublished - 15 May 2002