An in vitro model for the development of mature bone containing an osteocyte network

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An in vitro model for the development of mature bone containing an osteocyte network. / Iordachescu, Alexandra; Amin, Harsh; Rankin, Sara; Williams, Richard; Yapp, Clarence ; Bannerman, Alistair; Pacureanu, Alexandra; Addison, Owen; Hulley, Philippa; Grover, Liam.

In: Advanced Biosystems, Vol. 2, No. 2, 1700156, 02.2018.

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Iordachescu, Alexandra ; Amin, Harsh ; Rankin, Sara ; Williams, Richard ; Yapp, Clarence ; Bannerman, Alistair ; Pacureanu, Alexandra ; Addison, Owen ; Hulley, Philippa ; Grover, Liam. / An in vitro model for the development of mature bone containing an osteocyte network. In: Advanced Biosystems. 2018 ; Vol. 2, No. 2.

Bibtex

@article{eab649b091234a209057986d4d9b1812,
title = "An in vitro model for the development of mature bone containing an osteocyte network",
abstract = "Bone is a dynamic tissue that remodels continuously in response to local mechanical and chemical stimuli. This process can also result in maladaptive ectopic bone in response to injury, yet pathological differences at the molecular and structural levels are poorly understood. A number of in vivo models exist but can often be too complex to allow isolation of factors which may stimulate disease progression. A self-structuring model of bone formation is presented using a fibrin gel cast between two calcium phosphate ceramic anchors. Femoral periosteal cells, seeded into these structures, deposit an ordered matrix that closely resembles mature bone in terms of chemistry (collagen:mineral ratio) and structure, which is adapted over a period of one year in culture. Raman spectroscopy and X-ray diffraction confirm that the mineral is hydroxyapatite associated with collagen. Second-harmonic imaging demonstrates that collagen is organized similarly to mature mouse femora. Remarkably, cells differentiated to the osteocyte phase are linked by canaliculi (as demonstrated with nano-computed tomography) and remained viable over the full year of culture. It is demonstrated that novel drugs can prevent ossification in constructs. This model can be employed to study bone formation in an effort to encourage or prevent ossification in a range of pathologies.",
author = "Alexandra Iordachescu and Harsh Amin and Sara Rankin and Richard Williams and Clarence Yapp and Alistair Bannerman and Alexandra Pacureanu and Owen Addison and Philippa Hulley and Liam Grover",
year = "2018",
month = feb,
doi = "10.1002/adbi.201700156",
language = "English",
volume = "2",
journal = "Advanced Biosystems",
issn = "2366-7478",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "2",

}

RIS

TY - JOUR

T1 - An in vitro model for the development of mature bone containing an osteocyte network

AU - Iordachescu, Alexandra

AU - Amin, Harsh

AU - Rankin, Sara

AU - Williams, Richard

AU - Yapp, Clarence

AU - Bannerman, Alistair

AU - Pacureanu, Alexandra

AU - Addison, Owen

AU - Hulley, Philippa

AU - Grover, Liam

PY - 2018/2

Y1 - 2018/2

N2 - Bone is a dynamic tissue that remodels continuously in response to local mechanical and chemical stimuli. This process can also result in maladaptive ectopic bone in response to injury, yet pathological differences at the molecular and structural levels are poorly understood. A number of in vivo models exist but can often be too complex to allow isolation of factors which may stimulate disease progression. A self-structuring model of bone formation is presented using a fibrin gel cast between two calcium phosphate ceramic anchors. Femoral periosteal cells, seeded into these structures, deposit an ordered matrix that closely resembles mature bone in terms of chemistry (collagen:mineral ratio) and structure, which is adapted over a period of one year in culture. Raman spectroscopy and X-ray diffraction confirm that the mineral is hydroxyapatite associated with collagen. Second-harmonic imaging demonstrates that collagen is organized similarly to mature mouse femora. Remarkably, cells differentiated to the osteocyte phase are linked by canaliculi (as demonstrated with nano-computed tomography) and remained viable over the full year of culture. It is demonstrated that novel drugs can prevent ossification in constructs. This model can be employed to study bone formation in an effort to encourage or prevent ossification in a range of pathologies.

AB - Bone is a dynamic tissue that remodels continuously in response to local mechanical and chemical stimuli. This process can also result in maladaptive ectopic bone in response to injury, yet pathological differences at the molecular and structural levels are poorly understood. A number of in vivo models exist but can often be too complex to allow isolation of factors which may stimulate disease progression. A self-structuring model of bone formation is presented using a fibrin gel cast between two calcium phosphate ceramic anchors. Femoral periosteal cells, seeded into these structures, deposit an ordered matrix that closely resembles mature bone in terms of chemistry (collagen:mineral ratio) and structure, which is adapted over a period of one year in culture. Raman spectroscopy and X-ray diffraction confirm that the mineral is hydroxyapatite associated with collagen. Second-harmonic imaging demonstrates that collagen is organized similarly to mature mouse femora. Remarkably, cells differentiated to the osteocyte phase are linked by canaliculi (as demonstrated with nano-computed tomography) and remained viable over the full year of culture. It is demonstrated that novel drugs can prevent ossification in constructs. This model can be employed to study bone formation in an effort to encourage or prevent ossification in a range of pathologies.

U2 - 10.1002/adbi.201700156

DO - 10.1002/adbi.201700156

M3 - Article

VL - 2

JO - Advanced Biosystems

JF - Advanced Biosystems

SN - 2366-7478

IS - 2

M1 - 1700156

ER -