An improved conjugation method for Pseudomonas syringae

Research output: Contribution to journalArticlepeer-review

Authors

  • Michelle Hulin
  • Helen Neale
  • Richard Harrison
  • John Mansfield
  • Dawn Arnold

Colleges, School and Institutes

External organisations

  • University of the West of England
  • NIAB EMR
  • Imperial College London
  • Harper Adams University

Abstract

In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

Details

Original languageEnglish
JournalJournal of Microbiological Methods
Publication statusAccepted/In press - 5 Aug 2020