An important role for Myb-MuvB and its target gene KIF23 in a mouse model of lung adenocarcinoma

Research output: Contribution to journalArticle

Authors

  • Fabian Iltzsche
  • Katja Simon
  • Sabine Stopp
  • Grit Pattschull
  • Sven Francke
  • Patrick Wolter
  • Stefanie Hauser
  • Daniel Murphy
  • Andreas Rosenwald
  • Stefan Gaubatz

Colleges, School and Institutes

External organisations

  • University of Wuerzburg, Wuerzburg, Germany
  • University of Glasgow

Abstract

The conserved Myb-MuvB (MMB) multiprotein complex has an important role in transcriptional activation of mitotic genes. MMB target genes are overexpressed in several different cancer types and their elevated expression is associated with an advanced tumor state and a poor prognosis. This suggests that MMB could contribute to tumorigenesis by mediating overexpression of mitotic genes. However, although MMB has been extensively characterized biochemically, the requirement for MMB in tumorigenesis in vivo has not been investigated. Here we demonstrate that MMB is required for tumor formation in a mouse model of lung cancer driven by oncogenic K-RAS. We also identify a requirement for the mitotic kinesin KIF23, a key target gene of MMB, in tumorigenesis. RNA interference-mediated depletion of KIF23 inhibited lung tumor formation in vivo and induced apoptosis in lung cancer cell lines. Our results suggest that inhibition of KIF23 could be a strategy for treatment of lung cancer.

Details

Original languageEnglish
JournalOncogene
Early online date23 May 2016
Publication statusE-pub ahead of print - 23 May 2016