An evaluation of measured and calculated serum free cortisol in a group of patients with known adrenal suppression

Research output: Contribution to journalArticle


  • NL Barlow
  • J Holme
  • Robert Stockley
  • Penelope Clark

Colleges, School and Institutes


BACKGROUND: Since more than 90% of cortisol is bound to protein, serum free cortisol (SFC) may be a more appropriate marker of adrenal status than total cortisol. However, measurement of SFC is technically difficult and calculated SFC may offer a more practical alternative. METHODS: SFC, measured by equilibrium dialysis coupled with immunoassay, and calculated using Coolens' equation from total cortisol and corticosteroid binding globulin (CBG) concentrations, was compared in short Synacthen test (SST) serum from 42 patients, of whom 20 demonstrated a suppressed adrenal response. RESULTS: Considering the patient group as a whole, calculated SFC was found to be significantly lower than measured SFC, pre- and post-Synacthen (P <0.05 and


Original languageEnglish
Pages (from-to)200-204
Number of pages5
JournalAnnals of Clinical Biochemistry
Issue number3
Publication statusPublished - 1 May 2010