An essential role for the IL-7 receptor during intrathymic expansion of the positively selected neonatal T cell repertoire

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@article{fba19ca06b8f4346815920f30729c682,
title = "An essential role for the IL-7 receptor during intrathymic expansion of the positively selected neonatal T cell repertoire",
abstract = "Intrathymic T cell development is a multistage process involving discrete phases of proliferation as well as differentiation. From studies on IL-7 or IL-7R alpha -deficient mice, it is clear that the IL-7 receptor (IL-7R) plays a critical role during the initial stages of intrathymic CD4(-)8(-) precursor development. In contrast, the role of IL-7R in later stages of thymocyte development are unclear. Here, we have used various approaches to investigate directly the role of the IL-7R in thymocyte positive selection and the recently described phase of postselection proliferation. First, we show that positive selection involves selective up-regulation of IL-7R alpha- and IL-7R gamma -chains, with the majority of CD4(+) and CD8(+) cells being IL-7R(+), Second, MHC class II+ thymic epithelium-which drives postselection proliferation-expresses IL-7 mRNA, Finally, analysis of positive selection and postselection proliferation in thymocytes from IL-7R alpha (-/-) neonates shows that positive selection occurs normally, whereas postselection expansion is drastically reduced. Thus, our data provide the first evidence that, as well as playing a role during early phases of thymic development, IL-7R mediates intrathymic expansion of positively selected thymocytes, which may aid in establishment of the neonatal peripheral T cell pool.",
author = "Katherine Hare and Eric Jenkinson and Graham Anderson",
year = "2000",
month = jan,
day = "1",
language = "English",
volume = "165",
pages = "2410--4",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

RIS

TY - JOUR

T1 - An essential role for the IL-7 receptor during intrathymic expansion of the positively selected neonatal T cell repertoire

AU - Hare, Katherine

AU - Jenkinson, Eric

AU - Anderson, Graham

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Intrathymic T cell development is a multistage process involving discrete phases of proliferation as well as differentiation. From studies on IL-7 or IL-7R alpha -deficient mice, it is clear that the IL-7 receptor (IL-7R) plays a critical role during the initial stages of intrathymic CD4(-)8(-) precursor development. In contrast, the role of IL-7R in later stages of thymocyte development are unclear. Here, we have used various approaches to investigate directly the role of the IL-7R in thymocyte positive selection and the recently described phase of postselection proliferation. First, we show that positive selection involves selective up-regulation of IL-7R alpha- and IL-7R gamma -chains, with the majority of CD4(+) and CD8(+) cells being IL-7R(+), Second, MHC class II+ thymic epithelium-which drives postselection proliferation-expresses IL-7 mRNA, Finally, analysis of positive selection and postselection proliferation in thymocytes from IL-7R alpha (-/-) neonates shows that positive selection occurs normally, whereas postselection expansion is drastically reduced. Thus, our data provide the first evidence that, as well as playing a role during early phases of thymic development, IL-7R mediates intrathymic expansion of positively selected thymocytes, which may aid in establishment of the neonatal peripheral T cell pool.

AB - Intrathymic T cell development is a multistage process involving discrete phases of proliferation as well as differentiation. From studies on IL-7 or IL-7R alpha -deficient mice, it is clear that the IL-7 receptor (IL-7R) plays a critical role during the initial stages of intrathymic CD4(-)8(-) precursor development. In contrast, the role of IL-7R in later stages of thymocyte development are unclear. Here, we have used various approaches to investigate directly the role of the IL-7R in thymocyte positive selection and the recently described phase of postselection proliferation. First, we show that positive selection involves selective up-regulation of IL-7R alpha- and IL-7R gamma -chains, with the majority of CD4(+) and CD8(+) cells being IL-7R(+), Second, MHC class II+ thymic epithelium-which drives postselection proliferation-expresses IL-7 mRNA, Finally, analysis of positive selection and postselection proliferation in thymocytes from IL-7R alpha (-/-) neonates shows that positive selection occurs normally, whereas postselection expansion is drastically reduced. Thus, our data provide the first evidence that, as well as playing a role during early phases of thymic development, IL-7R mediates intrathymic expansion of positively selected thymocytes, which may aid in establishment of the neonatal peripheral T cell pool.

M3 - Article

C2 - 10946265

VL - 165

SP - 2410

EP - 2414

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -