An autonomous molecular assembler for programmable chemical synthesis

Research output: Contribution to journalArticlepeer-review

Standard

An autonomous molecular assembler for programmable chemical synthesis. / Meng, Wenjing; Muscat, Richard A; McKee, Mireya L; Milnes, Phillip J; El-Sagheer, Afaf H; Bath, Jonathan; Davis, Benjamin G; Brown, Tom; O'Reilly, Rachel K; Turberfield, Andrew J.

In: Nature Chemistry, Vol. 8, No. 6, 11.04.2016, p. 542-548.

Research output: Contribution to journalArticlepeer-review

Harvard

Meng, W, Muscat, RA, McKee, ML, Milnes, PJ, El-Sagheer, AH, Bath, J, Davis, BG, Brown, T, O'Reilly, RK & Turberfield, AJ 2016, 'An autonomous molecular assembler for programmable chemical synthesis', Nature Chemistry, vol. 8, no. 6, pp. 542-548. https://doi.org/10.1038/nchem.2495

APA

Meng, W., Muscat, R. A., McKee, M. L., Milnes, P. J., El-Sagheer, A. H., Bath, J., Davis, B. G., Brown, T., O'Reilly, R. K., & Turberfield, A. J. (2016). An autonomous molecular assembler for programmable chemical synthesis. Nature Chemistry, 8(6), 542-548. https://doi.org/10.1038/nchem.2495

Vancouver

Meng W, Muscat RA, McKee ML, Milnes PJ, El-Sagheer AH, Bath J et al. An autonomous molecular assembler for programmable chemical synthesis. Nature Chemistry. 2016 Apr 11;8(6):542-548. https://doi.org/10.1038/nchem.2495

Author

Meng, Wenjing ; Muscat, Richard A ; McKee, Mireya L ; Milnes, Phillip J ; El-Sagheer, Afaf H ; Bath, Jonathan ; Davis, Benjamin G ; Brown, Tom ; O'Reilly, Rachel K ; Turberfield, Andrew J. / An autonomous molecular assembler for programmable chemical synthesis. In: Nature Chemistry. 2016 ; Vol. 8, No. 6. pp. 542-548.

Bibtex

@article{81d1287641c84ac4a9eb7a991d0c66d6,
title = "An autonomous molecular assembler for programmable chemical synthesis",
abstract = "Molecular machines that assemble polymers in a programmed sequence are fundamental to life. They are also an achievable goal of nanotechnology. Here, we report synthetic molecular machinery made from DNA that controls and records the formation of covalent bonds. We show that an autonomous cascade of DNA hybridization reactions can create oligomers, from building blocks linked by olefin or peptide bonds, with a sequence defined by a reconfigurable molecular program. The system can also be programmed to achieve combinatorial assembly. The sequence of assembly reactions and thus the structure of each oligomer synthesized is recorded in a DNA molecule, which enables this information to be recovered by PCR amplification followed by DNA sequencing.",
keywords = "DNA/chemical synthesis, Genetic Engineering/methods, Models, Molecular, Molecular Structure, Nanostructures/chemistry, Nanotechnology/methods, Nucleic Acid Hybridization/methods, Oligonucleotides/chemical synthesis, Polymerization, Polymers/chemistry",
author = "Wenjing Meng and Muscat, {Richard A} and McKee, {Mireya L} and Milnes, {Phillip J} and El-Sagheer, {Afaf H} and Jonathan Bath and Davis, {Benjamin G} and Tom Brown and O'Reilly, {Rachel K} and Turberfield, {Andrew J}",
year = "2016",
month = apr,
day = "11",
doi = "10.1038/nchem.2495",
language = "English",
volume = "8",
pages = "542--548",
journal = "Nature Chemistry",
issn = "1755-4330",
publisher = "Nature Publishing Group",
number = "6",

}

RIS

TY - JOUR

T1 - An autonomous molecular assembler for programmable chemical synthesis

AU - Meng, Wenjing

AU - Muscat, Richard A

AU - McKee, Mireya L

AU - Milnes, Phillip J

AU - El-Sagheer, Afaf H

AU - Bath, Jonathan

AU - Davis, Benjamin G

AU - Brown, Tom

AU - O'Reilly, Rachel K

AU - Turberfield, Andrew J

PY - 2016/4/11

Y1 - 2016/4/11

N2 - Molecular machines that assemble polymers in a programmed sequence are fundamental to life. They are also an achievable goal of nanotechnology. Here, we report synthetic molecular machinery made from DNA that controls and records the formation of covalent bonds. We show that an autonomous cascade of DNA hybridization reactions can create oligomers, from building blocks linked by olefin or peptide bonds, with a sequence defined by a reconfigurable molecular program. The system can also be programmed to achieve combinatorial assembly. The sequence of assembly reactions and thus the structure of each oligomer synthesized is recorded in a DNA molecule, which enables this information to be recovered by PCR amplification followed by DNA sequencing.

AB - Molecular machines that assemble polymers in a programmed sequence are fundamental to life. They are also an achievable goal of nanotechnology. Here, we report synthetic molecular machinery made from DNA that controls and records the formation of covalent bonds. We show that an autonomous cascade of DNA hybridization reactions can create oligomers, from building blocks linked by olefin or peptide bonds, with a sequence defined by a reconfigurable molecular program. The system can also be programmed to achieve combinatorial assembly. The sequence of assembly reactions and thus the structure of each oligomer synthesized is recorded in a DNA molecule, which enables this information to be recovered by PCR amplification followed by DNA sequencing.

KW - DNA/chemical synthesis

KW - Genetic Engineering/methods

KW - Models, Molecular

KW - Molecular Structure

KW - Nanostructures/chemistry

KW - Nanotechnology/methods

KW - Nucleic Acid Hybridization/methods

KW - Oligonucleotides/chemical synthesis

KW - Polymerization

KW - Polymers/chemistry

U2 - 10.1038/nchem.2495

DO - 10.1038/nchem.2495

M3 - Article

C2 - 27219697

VL - 8

SP - 542

EP - 548

JO - Nature Chemistry

JF - Nature Chemistry

SN - 1755-4330

IS - 6

ER -