An autonomous molecular assembler for programmable chemical synthesis

Research output: Contribution to journalArticlepeer-review

Authors

  • Wenjing Meng
  • Richard A Muscat
  • Mireya L McKee
  • Phillip J Milnes
  • Afaf H El-Sagheer
  • Jonathan Bath
  • Benjamin G Davis
  • Tom Brown
  • Andrew J Turberfield

Colleges, School and Institutes

External organisations

  • Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK.
  • Department of Chemistry, University of Warwick, Coventry, West Midlands CV4 7AL, UK.
  • Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt.
  • Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK.
  • Department of Chemistry, The University of Warwick , Gibbet Hill Road, Coventry CV4 7AL, United Kingdom.

Abstract

Molecular machines that assemble polymers in a programmed sequence are fundamental to life. They are also an achievable goal of nanotechnology. Here, we report synthetic molecular machinery made from DNA that controls and records the formation of covalent bonds. We show that an autonomous cascade of DNA hybridization reactions can create oligomers, from building blocks linked by olefin or peptide bonds, with a sequence defined by a reconfigurable molecular program. The system can also be programmed to achieve combinatorial assembly. The sequence of assembly reactions and thus the structure of each oligomer synthesized is recorded in a DNA molecule, which enables this information to be recovered by PCR amplification followed by DNA sequencing.

Details

Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalNature Chemistry
Volume8
Issue number6
Publication statusPublished - 11 Apr 2016

Keywords

  • DNA/chemical synthesis, Genetic Engineering/methods, Models, Molecular, Molecular Structure, Nanostructures/chemistry, Nanotechnology/methods, Nucleic Acid Hybridization/methods, Oligonucleotides/chemical synthesis, Polymerization, Polymers/chemistry