An atlas of active enhancers across human cell types and tissues
Research output: Contribution to journal › Article
Colleges, School and Institutes
Enhancers control the correct temporal and cell-type-specific activation of gene expression in multicellular eukaryotes. Knowing their properties, regulatory activity and targets is crucial to understand the regulation of differentiation and homeostasis. Here we use the FANTOM5 panel of samples, covering the majority of human tissues and cell types, to produce an atlas of active, in vivo-transcribed enhancers. We show that enhancers share properties with CpG-poor messenger RNA promoters but produce bidirectional, exosome-sensitive, relatively short unspliced RNAs, the generation of which is strongly related to enhancer activity. The atlas is used to compare regulatory programs between different cells at unprecedented depth, to identify disease-associated regulatory single nucleotide polymorphisms, and to classify cell-type-specific and ubiquitous enhancers. We further explore the utility of enhancer redundancy, which explains gene expression strength rather than expression patterns. The online FANTOM5 enhancer atlas represents a unique resource for studies on cell-type-specific enhancers and gene regulation.
|Number of pages||7|
|Publication status||Published - 27 Mar 2014|
- Atlases as Topic, Cell Line, Cells, Cultured, Cluster Analysis, Enhancer Elements, Genetic, Gene Expression Regulation, Genetic Predisposition to Disease, HeLa Cells, Humans, Molecular Sequence Annotation, Organ Specificity, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, RNA, Messenger, Transcription Initiation Site, Transcription Initiation, Genetic