An adaptable analysis workflow for characterization of platelet spreading and morphology

Research output: Contribution to journalArticle

Abstract

The assessment of platelet spreading through light microscopy, and the subsequent quantification of parameters such as surface area and circularity, is a key assay for many platelet biologists. Here we present an analysis workflow which robustly segments individual platelets to facilitate the analysis of large numbers of cells while minimizing user bias. Image segmentation is performed by interactive learning and touching platelets are separated with an efficient semi-automated protocol. We also use machine learning methods to robustly automate the classification of platelets into different subtypes. These adaptable and reproducible workflows are made freely available and are implemented using the open-source software KNIME and ilastik.

Details

Original languageEnglish
JournalPlatelets
Early online date23 Apr 2020
Publication statusE-pub ahead of print - 23 Apr 2020

Keywords

  • Image analysis, machine learning, platelets, spreading