Altered SMRT levels disrupt vitamin D3 receptor signalling in prostate cancer cells

Research output: Contribution to journalArticle

Abstract

We hypothesized that key antiproliferative target genes for the vitamin D receptor (VDR) were repressed by an epigenetic mechanism in prostate cancer cells resulting in apparent hormonal insensitivity. To explore this possibility, we examined nuclear receptor corepressor expression in a panel of nonmalignant and malignant cell lines and primary cultures, and found frequently elevated SMRT corepressor mRNA expression often associated with reduced sensitivity to 1alpha,25-dihydroxyvitamin D(3) (1alpha,25(OH)2D3). For example, PC-3 and DU-145 prostate cancer cell lines had 1.8-fold and twofold increases in SMRT mRNA relative to normal PrEC cells (P

Details

Original languageEnglish
Pages (from-to)6712-25
Number of pages14
JournalOncogene
Volume23
Early online date9 Aug 2004
Publication statusPublished - 9 Aug 2004

Keywords

  • 1 alpha,25-dihydroxyvitamin D-3, histone deacetylation inhibitors, prostate cancer, SMRT, GADD45 alpha