Alginate/protamine/silica hybrid capsules with ultrathin membranes for laccase immobilization

Research output: Contribution to journalArticle

Authors

  • Ji Yun Wang
  • Hai Rong Yu
  • Rui Xie
  • Xiao Jie Ju
  • Ya Lan Yu
  • Liang Yin Chu

Colleges, School and Institutes

External organisations

  • Sichuan University
  • Chengdu Rongsheng Pharmaceuticals Co. Ltd.

Abstract

A novel type of core-shell capsules with ultrathin alginate/protamine/silica (APSi) hybrid membranes are successfully fabricated through a coextrusion minifluidic approach and a biosilicification method for immobilization of laccase. The ultrathin membranes were beneficial to the mass transfer across the capsule membranes, and the silica layer on the outer surface was efficient to inhibit the swelling of the capsule membranes. The immobilizing yield was considered to be 100% because all the enzyme molecules were encapsulated inside the capsules through the proposed method, and the laccase activity immobilized in APSi capsules was 61.8 mmol·g-1·min-1. The thermal, pH and storage stabilities of the immobilized laccase in APSi capsules were determined in comparison with free laccase. The stability of encapsulated laccase was significantly improved, which was as high as 67% after 20 days. The residual relative activity of encapsulated laccase remained 45% after 10 cycles.

Details

Original languageEnglish
Pages (from-to)380-389
Number of pages10
JournalAIChE Journal
Volume59
Issue number2
Publication statusPublished - Feb 2013

Keywords

  • Alginate/protamine/silica hybrid capsules, Biomimetic capsules, Enzyme immobilization, Laccase, Silicification