Adipocyte-specific deletion of IL-6 does not attenuate obesity-induced weight gain or glucose intolerance in mice

Research output: Contribution to journalArticlepeer-review

Authors

  • Martin Pal
  • Tim Petzold
  • Marit Hjorth
  • Casey L Egan
  • Julia S Brunner
  • Emma Estevez
  • Peter Iliades
  • Borivoj Zivanovic
  • Saskia Reibe
  • William E Hughes
  • Maria Findeisen
  • Juan Hidalgo
  • Mark A Febbraio

Colleges, School and Institutes

External organisations

  • Garvan Institute of Medical Research
  • Medical University of Vienna
  • Baker Heart and Diabetes Institute
  • University of New South Wales
  • Universitat Autonoma de Barcelona
  • Monash University

Abstract

OBJECTIVES: It has been suggested that interleukin-6 (IL-6) produced by adipocytes in obesity leads to liver insulin resistance, although this hypothesis has never been definitively tested. Accordingly, we did so by generating adipocyte specific IL-6 deficient (AdipoIL-6-/-) mice and studying them in the context of diet-induced and genetic obesity.

METHODS: Mice carrying 2 floxed alleles of IL-6 (C57Bl/6J) were crossed with Cre recombinase overexpressing mice driven by the adiponectin promoter to generate AdipoIL-6-/- mice. AdipoIL-6-/- and floxed littermate controls (FL) were fed a standard chow (CHOW) or high fat diet (HFD) for 16 weeks and comprehensively metabolically phenotyped. In addition to a diet-induced obesity (DIO) model, we also examined the role of adipocyte derived IL-6 in a genetic model of obesity and insulin resistance by crossing the AdipoIL-6-/- mice with leptin deficient (ob/ob) mice.

RESULTS: As expected, mice on a HFD and ob/ob mice displayed marked weight gain and increased fat mass compared with chow fed and ob/+ (littermate control) animals, respectively. However, deletion of IL-6 from adipocytes in either model had no effect on glucose tolerance or fasting hyperinsulinemia.

CONCLUSION: Adipocyte specific IL-6 does not contribute to whole body glucose intolerance in obese mice.

Details

Original languageEnglish
Pages (from-to)E597-E604
Number of pages8
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume317
Issue number4
Early online date6 Aug 2019
Publication statusPublished - 1 Oct 2019

Keywords

  • Glucose tolerance, Adiposity, Floxed, IL6, Obesity, adiposity, floxed, glucose tolerance, IL-6, obesity

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