Adherence of oral streptococci to nanostructured titanium surfaces

Krunal Narendrakumar; Mukta Kulkarni; Owen Addison; Anca Mazare; Ita Junkar; Patrik Schmuki; Rachel Sammons; Aleš Iglič

doi:10.1016/j.dental.2015.09.011

Adherence of oral streptococci to nanostructured titanium surfaces

Krunal Narendrakumar, Mukta Kulkarni, Owen Addison, Anca Mazare, Ita Junkar, Patrik Schmuki, Rachel Sammons, Aleš Iglič

Dentistry

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

265 Downloads (Pure)

Abstract

OBJECTIVES: Peri-implantitis and peri-mucositis pose a severe threat to the success of dental implants. Current research focuses on the development of surfaces that inhibit biofilm formation while not inferring with tissue integration. This study compared the adherence of two oral bacterial species, Streptococcus sanguinis and Streptococcus mutans to nanostructured titanium surfaces.

METHODS: The samples included TiO2 nanotubes formed by anodization of titanium foil of 100, 50 and 15nm diameter (NT15, NT50, NT100), a nanoporous (15nm pore diameter) surface and compact TiO2 control. Adherent surviving bacteria were enumerated after 1h in an artificial saliva medium containing bovine mucin.

RESULTS: Lowest numbers of adherent bacteria of both species were recovered from the original titanium foil and nanoporous surface and highest numbers from the Ti100 nanotubes. Numbers of attached S. sanguinis increased in the order (NT15<NT50<NT100), correlated with increasing percentage of surface fluoride. The lowest adhesion of S. sanguinis and S. mutans on TiO2 nanostructured surfaces was observed for small diameter nanoporous surfaces which coincides with the highest osteoblast adhesion on small diameter nanotubular/nanoporous surfaces shown in previous work.

SIGNIFICANCE: This study indicates that the adherence of oral streptococci can be modified by titanium anodization and nanotube diameter.

Original language	English
Pages (from-to)	1460-1468
Journal	Dental Materials
Volume	31
Issue number	12
Early online date	23 Oct 2015
DOIs	https://doi.org/10.1016/j.dental.2015.09.011
Publication status	Published - Dec 2015

Keywords

TiO2
Anodization
Nanotexture
Dental implant
Antimicrobial
Peri-implantitis

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10.1016/j.dental.2015.09.011Licence: Creative Commons: Attribution (CC BY)

Adherence of oral stretptococci to Nanostructured titanium
Checked for eligibility: 06/01/2016
Final published version, 1.18 MBLicence: Creative Commons: Attribution (CC BY)

Modification of the inflammatory response in peri-implant sites by titanium ions and debris
Addison, O., Chapple, I. & Cooper, P.
NIHR
1/03/11 → 29/02/16
Project: Other Government Departments

Cite this

@article{6474e1279ebd42a68debfebc3fdc9ebb,

title = "Adherence of oral streptococci to nanostructured titanium surfaces",

abstract = "OBJECTIVES: Peri-implantitis and peri-mucositis pose a severe threat to the success of dental implants. Current research focuses on the development of surfaces that inhibit biofilm formation while not inferring with tissue integration. This study compared the adherence of two oral bacterial species, Streptococcus sanguinis and Streptococcus mutans to nanostructured titanium surfaces.METHODS: The samples included TiO2 nanotubes formed by anodization of titanium foil of 100, 50 and 15nm diameter (NT15, NT50, NT100), a nanoporous (15nm pore diameter) surface and compact TiO2 control. Adherent surviving bacteria were enumerated after 1h in an artificial saliva medium containing bovine mucin.RESULTS: Lowest numbers of adherent bacteria of both species were recovered from the original titanium foil and nanoporous surface and highest numbers from the Ti100 nanotubes. Numbers of attached S. sanguinis increased in the order (NT15<NT50<NT100), correlated with increasing percentage of surface fluoride. The lowest adhesion of S. sanguinis and S. mutans on TiO2 nanostructured surfaces was observed for small diameter nanoporous surfaces which coincides with the highest osteoblast adhesion on small diameter nanotubular/nanoporous surfaces shown in previous work.SIGNIFICANCE: This study indicates that the adherence of oral streptococci can be modified by titanium anodization and nanotube diameter.",

keywords = "TiO2, Anodization, Nanotexture, Dental implant, Antimicrobial, Peri-implantitis",

author = "Krunal Narendrakumar and Mukta Kulkarni and Owen Addison and Anca Mazare and Ita Junkar and Patrik Schmuki and Rachel Sammons and Ale{\v s} Igli{\v c}",

year = "2015",

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doi = "10.1016/j.dental.2015.09.011",

language = "English",

volume = "31",

pages = "1460--1468",

journal = "Dental Materials",

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T1 - Adherence of oral streptococci to nanostructured titanium surfaces

AU - Narendrakumar, Krunal

AU - Kulkarni, Mukta

AU - Addison, Owen

AU - Mazare, Anca

AU - Junkar, Ita

AU - Schmuki, Patrik

AU - Sammons, Rachel

AU - Iglič, Aleš

PY - 2015/12

Y1 - 2015/12

N2 - OBJECTIVES: Peri-implantitis and peri-mucositis pose a severe threat to the success of dental implants. Current research focuses on the development of surfaces that inhibit biofilm formation while not inferring with tissue integration. This study compared the adherence of two oral bacterial species, Streptococcus sanguinis and Streptococcus mutans to nanostructured titanium surfaces.METHODS: The samples included TiO2 nanotubes formed by anodization of titanium foil of 100, 50 and 15nm diameter (NT15, NT50, NT100), a nanoporous (15nm pore diameter) surface and compact TiO2 control. Adherent surviving bacteria were enumerated after 1h in an artificial saliva medium containing bovine mucin.RESULTS: Lowest numbers of adherent bacteria of both species were recovered from the original titanium foil and nanoporous surface and highest numbers from the Ti100 nanotubes. Numbers of attached S. sanguinis increased in the order (NT15<NT50<NT100), correlated with increasing percentage of surface fluoride. The lowest adhesion of S. sanguinis and S. mutans on TiO2 nanostructured surfaces was observed for small diameter nanoporous surfaces which coincides with the highest osteoblast adhesion on small diameter nanotubular/nanoporous surfaces shown in previous work.SIGNIFICANCE: This study indicates that the adherence of oral streptococci can be modified by titanium anodization and nanotube diameter.

AB - OBJECTIVES: Peri-implantitis and peri-mucositis pose a severe threat to the success of dental implants. Current research focuses on the development of surfaces that inhibit biofilm formation while not inferring with tissue integration. This study compared the adherence of two oral bacterial species, Streptococcus sanguinis and Streptococcus mutans to nanostructured titanium surfaces.METHODS: The samples included TiO2 nanotubes formed by anodization of titanium foil of 100, 50 and 15nm diameter (NT15, NT50, NT100), a nanoporous (15nm pore diameter) surface and compact TiO2 control. Adherent surviving bacteria were enumerated after 1h in an artificial saliva medium containing bovine mucin.RESULTS: Lowest numbers of adherent bacteria of both species were recovered from the original titanium foil and nanoporous surface and highest numbers from the Ti100 nanotubes. Numbers of attached S. sanguinis increased in the order (NT15<NT50<NT100), correlated with increasing percentage of surface fluoride. The lowest adhesion of S. sanguinis and S. mutans on TiO2 nanostructured surfaces was observed for small diameter nanoporous surfaces which coincides with the highest osteoblast adhesion on small diameter nanotubular/nanoporous surfaces shown in previous work.SIGNIFICANCE: This study indicates that the adherence of oral streptococci can be modified by titanium anodization and nanotube diameter.

KW - TiO2

KW - Anodization

KW - Nanotexture

KW - Dental implant

KW - Antimicrobial

KW - Peri-implantitis

U2 - 10.1016/j.dental.2015.09.011

DO - 10.1016/j.dental.2015.09.011

M3 - Article

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SN - 0109-5641

VL - 31

SP - 1460

EP - 1468

JO - Dental Materials

JF - Dental Materials

IS - 12

ER -

Adherence of oral streptococci to nanostructured titanium surfaces

Abstract

Keywords

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Modification of the inflammatory response in peri-implant sites by titanium ions and debris

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