Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo

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Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo. / Iqbal, Asif J; Barrett, Tessa J; Taylor, Lewis; McNeill, Eileen; Manmadhan, Arun; Recio, Carlota; Carmineri, Alfredo; Brodermann, Maximillian H; White, Gemma E; Cooper, Dianne; DiDonato, Joseph A; Zamanian-Daryoush, Maryam; Hazen, Stanley L; Channon, Keith M; Greaves, David R; Fisher, Edward A.

In: Elife, Vol. 5, 30.08.2016.

Research output: Contribution to journalArticle

Harvard

Iqbal, AJ, Barrett, TJ, Taylor, L, McNeill, E, Manmadhan, A, Recio, C, Carmineri, A, Brodermann, MH, White, GE, Cooper, D, DiDonato, JA, Zamanian-Daryoush, M, Hazen, SL, Channon, KM, Greaves, DR & Fisher, EA 2016, 'Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo', Elife, vol. 5. https://doi.org/10.7554/eLife.15190

APA

Iqbal, A. J., Barrett, T. J., Taylor, L., McNeill, E., Manmadhan, A., Recio, C., Carmineri, A., Brodermann, M. H., White, G. E., Cooper, D., DiDonato, J. A., Zamanian-Daryoush, M., Hazen, S. L., Channon, K. M., Greaves, D. R., & Fisher, E. A. (2016). Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo. Elife, 5. https://doi.org/10.7554/eLife.15190

Vancouver

Author

Iqbal, Asif J ; Barrett, Tessa J ; Taylor, Lewis ; McNeill, Eileen ; Manmadhan, Arun ; Recio, Carlota ; Carmineri, Alfredo ; Brodermann, Maximillian H ; White, Gemma E ; Cooper, Dianne ; DiDonato, Joseph A ; Zamanian-Daryoush, Maryam ; Hazen, Stanley L ; Channon, Keith M ; Greaves, David R ; Fisher, Edward A. / Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo. In: Elife. 2016 ; Vol. 5.

Bibtex

@article{220c97015e05470ab216147874c632a3,
title = "Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo",
abstract = "Apolipoprotein A1 (apoA1) is the major protein component of high-density lipoprotein (HDL) and has well documented anti-inflammatory properties. To better understand the cellular and molecular basis of the anti-inflammatory actions of apoA1, we explored the effect of acute human apoA1 exposure on the migratory capacity of monocyte-derived cells in vitro and in vivo. Acute (20-60 min) apoA1 treatment induced a substantial (50-90%) reduction in macrophage chemotaxis to a range of chemoattractants. This acute treatment was anti-inflammatory in vivo as shown by pre-treatment of monocytes prior to adoptive transfer into an on-going murine peritonitis model. We find that apoA1 rapidly disrupts membrane lipid rafts, and as a consequence, dampens the PI3K/Akt signalling pathway that coordinates reorganization of the actin cytoskeleton and cell migration. Our data strengthen the evidence base for therapeutic apoA1 infusions in situations where reduced monocyte recruitment to sites of inflammation could have beneficial outcomes.",
keywords = "Journal Article",
author = "Iqbal, {Asif J} and Barrett, {Tessa J} and Lewis Taylor and Eileen McNeill and Arun Manmadhan and Carlota Recio and Alfredo Carmineri and Brodermann, {Maximillian H} and White, {Gemma E} and Dianne Cooper and DiDonato, {Joseph A} and Maryam Zamanian-Daryoush and Hazen, {Stanley L} and Channon, {Keith M} and Greaves, {David R} and Fisher, {Edward A}",
year = "2016",
month = aug
day = "30",
doi = "10.7554/eLife.15190",
language = "English",
volume = "5",
journal = "Elife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

RIS

TY - JOUR

T1 - Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo

AU - Iqbal, Asif J

AU - Barrett, Tessa J

AU - Taylor, Lewis

AU - McNeill, Eileen

AU - Manmadhan, Arun

AU - Recio, Carlota

AU - Carmineri, Alfredo

AU - Brodermann, Maximillian H

AU - White, Gemma E

AU - Cooper, Dianne

AU - DiDonato, Joseph A

AU - Zamanian-Daryoush, Maryam

AU - Hazen, Stanley L

AU - Channon, Keith M

AU - Greaves, David R

AU - Fisher, Edward A

PY - 2016/8/30

Y1 - 2016/8/30

N2 - Apolipoprotein A1 (apoA1) is the major protein component of high-density lipoprotein (HDL) and has well documented anti-inflammatory properties. To better understand the cellular and molecular basis of the anti-inflammatory actions of apoA1, we explored the effect of acute human apoA1 exposure on the migratory capacity of monocyte-derived cells in vitro and in vivo. Acute (20-60 min) apoA1 treatment induced a substantial (50-90%) reduction in macrophage chemotaxis to a range of chemoattractants. This acute treatment was anti-inflammatory in vivo as shown by pre-treatment of monocytes prior to adoptive transfer into an on-going murine peritonitis model. We find that apoA1 rapidly disrupts membrane lipid rafts, and as a consequence, dampens the PI3K/Akt signalling pathway that coordinates reorganization of the actin cytoskeleton and cell migration. Our data strengthen the evidence base for therapeutic apoA1 infusions in situations where reduced monocyte recruitment to sites of inflammation could have beneficial outcomes.

AB - Apolipoprotein A1 (apoA1) is the major protein component of high-density lipoprotein (HDL) and has well documented anti-inflammatory properties. To better understand the cellular and molecular basis of the anti-inflammatory actions of apoA1, we explored the effect of acute human apoA1 exposure on the migratory capacity of monocyte-derived cells in vitro and in vivo. Acute (20-60 min) apoA1 treatment induced a substantial (50-90%) reduction in macrophage chemotaxis to a range of chemoattractants. This acute treatment was anti-inflammatory in vivo as shown by pre-treatment of monocytes prior to adoptive transfer into an on-going murine peritonitis model. We find that apoA1 rapidly disrupts membrane lipid rafts, and as a consequence, dampens the PI3K/Akt signalling pathway that coordinates reorganization of the actin cytoskeleton and cell migration. Our data strengthen the evidence base for therapeutic apoA1 infusions in situations where reduced monocyte recruitment to sites of inflammation could have beneficial outcomes.

KW - Journal Article

U2 - 10.7554/eLife.15190

DO - 10.7554/eLife.15190

M3 - Article

C2 - 27572261

VL - 5

JO - Elife

JF - Elife

SN - 2050-084X

ER -