Activity of FCE 22101 against methicillin-resistant Staphylococcus aureus and affinity for penicillin binding proteins

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Activity of FCE 22101 against methicillin-resistant Staphylococcus aureus and affinity for penicillin binding proteins. / Piddock, L J; Traynor, E A; Wise, R.

In: Journal of Antimicrobial Chemotherapy, Vol. 23 Suppl C, 03.1989, p. 59-64.

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@article{d389e7d34d9b42608e24a66551bcdde3,
title = "Activity of FCE 22101 against methicillin-resistant Staphylococcus aureus and affinity for penicillin binding proteins",
abstract = "The susceptibility of 47 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) to FCE 22101, imipenem and methicillin was determined with Iso-Sensitest media, with or without NaCl and with incubation at 30 degrees and 37 degrees C and for 24 and 48 h. All strains had a MIC 8 mg/l of methicillin under at least one of the culture conditions. The MIC90 of FCE 22101 was 1 mg/l, and that of imipenem 16 mg/l. The affinity of FCE 22101 for the penicillin-binding proteins (PBPs) of five clinical isolates of MRSA and S. aureus 13136 p-m+ was examined in envelope preparations and whole cell assay under the growth conditions listed above. PBP 2' was detected in all MRSA, and the clinical isolates had an I50 of 4 mg/l FCE 22101. These in-vitro data suggest that FCE 22101 may be active against MRSA in clinical use.",
keywords = "Anti-Bacterial Agents, Bacterial Proteins, Carbapenems, Carrier Proteins, Hexosyltransferases, Imipenem, Methicillin, Microbial Sensitivity Tests, Muramoylpentapeptide Carboxypeptidase, Penicillin Resistance, Penicillin-Binding Proteins, Peptidyl Transferases, Staphylococcus aureus",
author = "Piddock, {L J} and Traynor, {E A} and R Wise",
year = "1989",
month = mar,
language = "English",
volume = "23 Suppl C",
pages = "59--64",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - Activity of FCE 22101 against methicillin-resistant Staphylococcus aureus and affinity for penicillin binding proteins

AU - Piddock, L J

AU - Traynor, E A

AU - Wise, R

PY - 1989/3

Y1 - 1989/3

N2 - The susceptibility of 47 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) to FCE 22101, imipenem and methicillin was determined with Iso-Sensitest media, with or without NaCl and with incubation at 30 degrees and 37 degrees C and for 24 and 48 h. All strains had a MIC 8 mg/l of methicillin under at least one of the culture conditions. The MIC90 of FCE 22101 was 1 mg/l, and that of imipenem 16 mg/l. The affinity of FCE 22101 for the penicillin-binding proteins (PBPs) of five clinical isolates of MRSA and S. aureus 13136 p-m+ was examined in envelope preparations and whole cell assay under the growth conditions listed above. PBP 2' was detected in all MRSA, and the clinical isolates had an I50 of 4 mg/l FCE 22101. These in-vitro data suggest that FCE 22101 may be active against MRSA in clinical use.

AB - The susceptibility of 47 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) to FCE 22101, imipenem and methicillin was determined with Iso-Sensitest media, with or without NaCl and with incubation at 30 degrees and 37 degrees C and for 24 and 48 h. All strains had a MIC 8 mg/l of methicillin under at least one of the culture conditions. The MIC90 of FCE 22101 was 1 mg/l, and that of imipenem 16 mg/l. The affinity of FCE 22101 for the penicillin-binding proteins (PBPs) of five clinical isolates of MRSA and S. aureus 13136 p-m+ was examined in envelope preparations and whole cell assay under the growth conditions listed above. PBP 2' was detected in all MRSA, and the clinical isolates had an I50 of 4 mg/l FCE 22101. These in-vitro data suggest that FCE 22101 may be active against MRSA in clinical use.

KW - Anti-Bacterial Agents

KW - Bacterial Proteins

KW - Carbapenems

KW - Carrier Proteins

KW - Hexosyltransferases

KW - Imipenem

KW - Methicillin

KW - Microbial Sensitivity Tests

KW - Muramoylpentapeptide Carboxypeptidase

KW - Penicillin Resistance

KW - Penicillin-Binding Proteins

KW - Peptidyl Transferases

KW - Staphylococcus aureus

M3 - Article

C2 - 2732144

VL - 23 Suppl C

SP - 59

EP - 64

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

ER -