Activation of σ-28-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization

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Colleges, School and Institutes

Abstract

The Escherichia coli aer regulatory region contains a single promoter that is recognized by RNA polymerase containing the flagellar sigma factor, σ28. Expression from this promoter is dependent on direct activation by the cyclic AMP receptor protein, which binds to a target centred 49.5 base pairs upstream from the transcript start. Activator-dependent transcription from the aer promoter was reconstituted in vitro, and a tethered inorganic nuclease was used to find the position of the C-terminal domains of the RNA polymerase α subunits in transcriptionally competent open complexes. We report that the ternary activator-RNA polymerase-aer promoter open complex is organized differently from complexes at previously characterized promoters. Among other E. coli promoters recognized by RNA polymerase containing σ28, only the trg promoter is activated directly by the cyclic AMP receptor protein. The organization of the different promoter elements and the activator binding site at the trg promoter is the same as at the aer promoter, suggesting a common activation mechanism.

Details

Original languageEnglish
Pages (from-to)1098-1111
Number of pages14
JournalMolecular Microbiology
Volume75
Issue number5
Early online date4 Nov 2009
Publication statusPublished - 1 Mar 2010