Activation by NarL at the Escherichia coli ogt promoter

Research output: Contribution to journalArticlepeer-review

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Activation by NarL at the Escherichia coli ogt promoter. / Ruanto, Patcharawarin; Chismon, David J; Hothersall, Joanne; Godfrey, Rita E; Lee, David J; Busby, Stephen J W; Browning, Douglas F.

In: The Biochemical journal, Vol. 477, No. 15, 14.08.2020, p. 2807-2820.

Research output: Contribution to journalArticlepeer-review

Harvard

Ruanto, P, Chismon, DJ, Hothersall, J, Godfrey, RE, Lee, DJ, Busby, SJW & Browning, DF 2020, 'Activation by NarL at the Escherichia coli ogt promoter', The Biochemical journal, vol. 477, no. 15, pp. 2807-2820. https://doi.org/10.1042/BCJ20200408

APA

Ruanto, P., Chismon, D. J., Hothersall, J., Godfrey, R. E., Lee, D. J., Busby, S. J. W., & Browning, D. F. (2020). Activation by NarL at the Escherichia coli ogt promoter. The Biochemical journal, 477(15), 2807-2820. https://doi.org/10.1042/BCJ20200408

Vancouver

Ruanto P, Chismon DJ, Hothersall J, Godfrey RE, Lee DJ, Busby SJW et al. Activation by NarL at the Escherichia coli ogt promoter. The Biochemical journal. 2020 Aug 14;477(15):2807-2820. https://doi.org/10.1042/BCJ20200408

Author

Ruanto, Patcharawarin ; Chismon, David J ; Hothersall, Joanne ; Godfrey, Rita E ; Lee, David J ; Busby, Stephen J W ; Browning, Douglas F. / Activation by NarL at the Escherichia coli ogt promoter. In: The Biochemical journal. 2020 ; Vol. 477, No. 15. pp. 2807-2820.

Bibtex

@article{2fdcef9c0d4747cfba672af27b837fc1,
title = "Activation by NarL at the Escherichia coli ogt promoter",
abstract = "The Escherichia coli NarX/NarL two-component response regulator system regulates gene expression in response to nitrate ions and the NarL protein is a global transcription factor, which activates transcript initiation at many target promoters. One such target, the E. coliogt promoter, which controls the expression of an O6‑alkylguanine-DNA-alkyltransferase, is dependent on NarL binding to two DNA targets centred at positions -44.5 and -77.5 upstream from the transcript start. Here, we describe ogt promoter derivatives that can be activated solely by NarL binding either at position -44.5 or position -77.5. We show that NarL can also activate the ogt promoter when located at position -67.5. We present data to argue that NarL-dependent activation of transcript initiation at the ogt promoter results from a direct interaction between NarL and a determinant in the C-terminal domain of the RNA polymerase α subunit. Footprinting experiments show that, at the -44.5 promoter, NarL and the C-terminal domain of the RNA polymerase α subunit bind to opposite faces of promoter DNA, suggesting an unusual mechanism of transcription activation. Our work suggests new organisations for activator-dependent transcription at promoters and future applications for biotechnology.",
keywords = "molecular interactions, NarL, promoters, transcription activation",
author = "Patcharawarin Ruanto and Chismon, {David J} and Joanne Hothersall and Godfrey, {Rita E} and Lee, {David J} and Busby, {Stephen J W} and Browning, {Douglas F}",
note = "{\textcopyright} 2020 The Author(s).",
year = "2020",
month = aug,
day = "14",
doi = "10.1042/BCJ20200408",
language = "English",
volume = "477",
pages = "2807--2820",
journal = "Biochem J",
issn = "0264-6021",
publisher = "Portland Press",
number = "15",

}

RIS

TY - JOUR

T1 - Activation by NarL at the Escherichia coli ogt promoter

AU - Ruanto, Patcharawarin

AU - Chismon, David J

AU - Hothersall, Joanne

AU - Godfrey, Rita E

AU - Lee, David J

AU - Busby, Stephen J W

AU - Browning, Douglas F

N1 - © 2020 The Author(s).

PY - 2020/8/14

Y1 - 2020/8/14

N2 - The Escherichia coli NarX/NarL two-component response regulator system regulates gene expression in response to nitrate ions and the NarL protein is a global transcription factor, which activates transcript initiation at many target promoters. One such target, the E. coliogt promoter, which controls the expression of an O6‑alkylguanine-DNA-alkyltransferase, is dependent on NarL binding to two DNA targets centred at positions -44.5 and -77.5 upstream from the transcript start. Here, we describe ogt promoter derivatives that can be activated solely by NarL binding either at position -44.5 or position -77.5. We show that NarL can also activate the ogt promoter when located at position -67.5. We present data to argue that NarL-dependent activation of transcript initiation at the ogt promoter results from a direct interaction between NarL and a determinant in the C-terminal domain of the RNA polymerase α subunit. Footprinting experiments show that, at the -44.5 promoter, NarL and the C-terminal domain of the RNA polymerase α subunit bind to opposite faces of promoter DNA, suggesting an unusual mechanism of transcription activation. Our work suggests new organisations for activator-dependent transcription at promoters and future applications for biotechnology.

AB - The Escherichia coli NarX/NarL two-component response regulator system regulates gene expression in response to nitrate ions and the NarL protein is a global transcription factor, which activates transcript initiation at many target promoters. One such target, the E. coliogt promoter, which controls the expression of an O6‑alkylguanine-DNA-alkyltransferase, is dependent on NarL binding to two DNA targets centred at positions -44.5 and -77.5 upstream from the transcript start. Here, we describe ogt promoter derivatives that can be activated solely by NarL binding either at position -44.5 or position -77.5. We show that NarL can also activate the ogt promoter when located at position -67.5. We present data to argue that NarL-dependent activation of transcript initiation at the ogt promoter results from a direct interaction between NarL and a determinant in the C-terminal domain of the RNA polymerase α subunit. Footprinting experiments show that, at the -44.5 promoter, NarL and the C-terminal domain of the RNA polymerase α subunit bind to opposite faces of promoter DNA, suggesting an unusual mechanism of transcription activation. Our work suggests new organisations for activator-dependent transcription at promoters and future applications for biotechnology.

KW - molecular interactions

KW - NarL, promoters

KW - transcription activation

UR - http://www.scopus.com/inward/record.url?scp=85089300915&partnerID=8YFLogxK

U2 - 10.1042/BCJ20200408

DO - 10.1042/BCJ20200408

M3 - Article

C2 - 32662815

VL - 477

SP - 2807

EP - 2820

JO - Biochem J

JF - Biochem J

SN - 0264-6021

IS - 15

ER -