Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

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Acrylamide-dT : a polymerisable nucleoside for DNA incorporation. / Allabush, Francia; Mendes, Paula M.; Tucker, James H. R.

In: RSC Advances, Vol. 9, No. 54, 03.10.2019, p. 31511-31516.

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@article{b61cad74826449478c2d9aa44b1f6b19,
title = "Acrylamide-dT: a polymerisable nucleoside for DNA incorporation",
abstract = "The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA–polymer hybrids for use in various macromolecular materials applications.",
author = "Francia Allabush and Mendes, {Paula M.} and Tucker, {James H. R.}",
year = "2019",
month = oct
day = "3",
doi = "10.1039/C9RA07570D",
language = "English",
volume = "9",
pages = "31511--31516",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "54",

}

RIS

TY - JOUR

T1 - Acrylamide-dT

T2 - a polymerisable nucleoside for DNA incorporation

AU - Allabush, Francia

AU - Mendes, Paula M.

AU - Tucker, James H. R.

PY - 2019/10/3

Y1 - 2019/10/3

N2 - The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA–polymer hybrids for use in various macromolecular materials applications.

AB - The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA–polymer hybrids for use in various macromolecular materials applications.

UR - http://www.scopus.com/inward/record.url?scp=85073265504&partnerID=8YFLogxK

U2 - 10.1039/C9RA07570D

DO - 10.1039/C9RA07570D

M3 - Article

VL - 9

SP - 31511

EP - 31516

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 54

ER -