Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

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Colleges, School and Institutes

Abstract

The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA–polymer hybrids for use in various macromolecular materials applications.

Details

Original languageEnglish
Pages (from-to)31511-31516
Number of pages6
JournalRSC Advances
Volume9
Issue number54
Publication statusPublished - 3 Oct 2019