Acetylcholine production by group 2 innate lymphoid cells promotes mucosal immunity to helminths

Research output: Contribution to journalArticlepeer-review

Authors

  • Luke B Roberts
  • Corinna Schnoeller
  • Rita Berkachy
  • Matthew Darby
  • Jamie Pillaye
  • Menno J Oudhoff
  • Naveen Parmar
  • Claire Mackowiak
  • Delphine Sedda
  • Valerie Quesniaux
  • Bernhard Ryffel
  • Rachel Vaux
  • Kleoniki Gounaris
  • Sylvie Berrard
  • Murray E Selkirk

External organisations

  • Wellcome Centre for Infectious Diseases Research in Africa
  • CNRS-University of Orleans
  • Le Studium Institute for Advanced Studies
  • Norwegian University of Science and Technology
  • University of Cape Town
  • Imperial College London
  • Université de Paris

Abstract

Innate lymphoid cells (ILCs) are critical mediators of immunological and physiological responses at mucosal barrier sites. Whereas neurotransmitters can stimulate ILCs, the synthesis of small-molecule neurotransmitters by these cells has only recently been appreciated. Group 2 ILCs (ILC2s) are shown here to synthesize and release acetylcholine (ACh) during parasitic nematode infection. The cholinergic phenotype of pulmonary ILC2s was associated with their activation state, could be induced by in vivo exposure to extracts of Alternaria alternata or the alarmin cytokines interleukin-33 (IL-33) and IL-25, and was augmented by IL-2 in vitro. Genetic disruption of ACh synthesis by murine ILC2s resulted in increased parasite burdens, lower numbers of ILC2s, and reduced lung and gut barrier responses to Nippostrongylus brasiliensis infection. These data demonstrate a functional role for ILC2-derived ACh in the expansion of ILC2s for maximal induction of type 2 immunity.

Details

Original languageEnglish
Article numbereabd0359
JournalScience Immunology
Volume6
Issue number57
Publication statusPublished - 5 Mar 2021

ASJC Scopus subject areas