Accumulation of KRM-1648 by Mycobacterium aurum and Mycobacterium tuberculosis

Research output: Contribution to journalArticle

Colleges, School and Institutes

Abstract

After exposure to 2 mg/L (14)C-labelled KRM-1648 (a new broad-spectrum benzoxazinorifamycin antibiotic) for 5 min, a steady-state concentration of 31.3 +/- 3 ng/mg cells KRM-1648 and 12. 6 +/- 0.3 ng/mg cells KRM-1648 was accumulated by wild-type antibiotic-susceptible Mycobacterium aurum (A+) and Mycobacterium tuberculosis (H37Rv), respectively. However, 2 mg/L KRM-1648 was bactericidal for M. tuberculosis. A steady-state concentration of 3. 7 +/- 0.1 ng/mg cells KRM-1648 was accumulated after exposure to 0.5 mg/L. At pH 4 higher concentrations were accumulated than at pH 7. A sub-inhibitory concentration of ethambutol increased the concentration of KRM-1648 accumulated, but Tween 80 and reserpine had little or no effect.

Details

Original languageEnglish
Pages (from-to)681-4
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume45
Issue number5
Publication statusPublished - May 2000

Keywords

  • Antibiotics, Antitubercular, Carbon Radioisotopes, Colony Count, Microbial, Ethambutol, Hydrogen-Ion Concentration, Microbial Sensitivity Tests, Mycobacterium, Mycobacterium tuberculosis, Polysorbates, Reserpine, Rifamycins