Accessible synthetic probes for staining actin inside platelets and megakaryocytes by employing Lifeact peptide

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@article{21738d73230849518ef5c2574a709954,
title = "Accessible synthetic probes for staining actin inside platelets and megakaryocytes by employing Lifeact peptide",
abstract = "Lifeact is a 17-residue peptide that can be employed in cell microscopy as a probe for F-actin when fused to fluorescent proteins, but therefore is not suitable for all cell types. We have conjugated fluorescently labelled Lifeact to three different cell-penetrating systems (a myristoylated carrier (myr), the pH low insertion peptide (pHLIP) and the cationic peptide TAT) as a strategy to deliver Lifeact into cells and developed new tools for actin staining with improved synthetic accessibility and low toxicity, focusing on their suitability in platelets and megakaryocytes. Using confocal microscopy, we characterised the cell distribution of the new hybrids in fixed cells, and found that both myr- and pHLIP-Lifeact conjugates provide efficient actin staining upon cleavage of Lifeact from the carriers, without affecting cell spreading. This new approach could facilitate the design of new tools for actin visualisation.",
keywords = "FRET, Lifeact, actin, cell-penetrating hybrids, fluorescent probes",
author = "Lucia Cardo and Steven Thomas and Alexandra Mazharian and Zoe Pikramenou and Joshua Rappoport and Michael Hannon and Steve Watson",
year = "2015",
month = jul,
day = "27",
doi = "10.1002/cbic.201500120",
language = "English",
volume = "16",
pages = "1680--1688",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "11",

}

RIS

TY - JOUR

T1 - Accessible synthetic probes for staining actin inside platelets and megakaryocytes by employing Lifeact peptide

AU - Cardo, Lucia

AU - Thomas, Steven

AU - Mazharian, Alexandra

AU - Pikramenou, Zoe

AU - Rappoport, Joshua

AU - Hannon, Michael

AU - Watson, Steve

PY - 2015/7/27

Y1 - 2015/7/27

N2 - Lifeact is a 17-residue peptide that can be employed in cell microscopy as a probe for F-actin when fused to fluorescent proteins, but therefore is not suitable for all cell types. We have conjugated fluorescently labelled Lifeact to three different cell-penetrating systems (a myristoylated carrier (myr), the pH low insertion peptide (pHLIP) and the cationic peptide TAT) as a strategy to deliver Lifeact into cells and developed new tools for actin staining with improved synthetic accessibility and low toxicity, focusing on their suitability in platelets and megakaryocytes. Using confocal microscopy, we characterised the cell distribution of the new hybrids in fixed cells, and found that both myr- and pHLIP-Lifeact conjugates provide efficient actin staining upon cleavage of Lifeact from the carriers, without affecting cell spreading. This new approach could facilitate the design of new tools for actin visualisation.

AB - Lifeact is a 17-residue peptide that can be employed in cell microscopy as a probe for F-actin when fused to fluorescent proteins, but therefore is not suitable for all cell types. We have conjugated fluorescently labelled Lifeact to three different cell-penetrating systems (a myristoylated carrier (myr), the pH low insertion peptide (pHLIP) and the cationic peptide TAT) as a strategy to deliver Lifeact into cells and developed new tools for actin staining with improved synthetic accessibility and low toxicity, focusing on their suitability in platelets and megakaryocytes. Using confocal microscopy, we characterised the cell distribution of the new hybrids in fixed cells, and found that both myr- and pHLIP-Lifeact conjugates provide efficient actin staining upon cleavage of Lifeact from the carriers, without affecting cell spreading. This new approach could facilitate the design of new tools for actin visualisation.

KW - FRET

KW - Lifeact

KW - actin

KW - cell-penetrating hybrids

KW - fluorescent probes

U2 - 10.1002/cbic.201500120

DO - 10.1002/cbic.201500120

M3 - Article

C2 - 26062886

VL - 16

SP - 1680

EP - 1688

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 11

ER -