Ability of IncP-6 plasmid pM3 to replicate in Escherichia coli is dependent on both rep and par functions

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Ability of IncP-6 plasmid pM3 to replicate in Escherichia coli is dependent on both rep and par functions. / Sevastsyanovich, Yanina; Titok, MA; Krasowiak, Renata; Bingle, Lewis; Thomas, Christopher.

In: Molecular Microbiology, Vol. 57, No. 3, 01.08.2005, p. 819-833.

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@article{d48796784aaf43d0a0e08a578c787620,
title = "Ability of IncP-6 plasmid pM3 to replicate in Escherichia coli is dependent on both rep and par functions",
abstract = "IncP-9 plasmids are common in Pseudomonas species and can be transferred to other Gram-negative eubacteria but tend not to be stably maintained outside their natural host genus. A 1.3 kb oriV-rep fragment from IncP-9 plasmid pM3 was sufficient for autonomous replication in Pseudomonas putida but not in Escherichia coli. Replication of oriV-rep in E. coli was restored when additional rep was provided in trans, suggesting that the replication defect resulted from insufficient rep expression from its natural promoter. A promoter deficiency in E. coli was confirmed by reporter gene assays, transcriptional start point mapping and mutation of the promoter recognition elements. Dissection of the pM3 mini-replicon, pMT2, showed that this replication deficiency in E. coli is suppressed by additional determinants from its par operon: ParB, which can be supplied in trans, and its target, the par operon promoter, required in cis to oriV-rep. We propose that ParB binding to its target either changes plasmid DNA and thus promoter conformation or by spreading or looping contacts RNAP at the rep promoter so that rep expression is sufficient to activate oriV.",
author = "Yanina Sevastsyanovich and MA Titok and Renata Krasowiak and Lewis Bingle and Christopher Thomas",
year = "2005",
month = aug,
day = "1",
doi = "10.1111/j.1365-2958.2005.04732.x",
language = "English",
volume = "57",
pages = "819--833",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley",
number = "3",

}

RIS

TY - JOUR

T1 - Ability of IncP-6 plasmid pM3 to replicate in Escherichia coli is dependent on both rep and par functions

AU - Sevastsyanovich, Yanina

AU - Titok, MA

AU - Krasowiak, Renata

AU - Bingle, Lewis

AU - Thomas, Christopher

PY - 2005/8/1

Y1 - 2005/8/1

N2 - IncP-9 plasmids are common in Pseudomonas species and can be transferred to other Gram-negative eubacteria but tend not to be stably maintained outside their natural host genus. A 1.3 kb oriV-rep fragment from IncP-9 plasmid pM3 was sufficient for autonomous replication in Pseudomonas putida but not in Escherichia coli. Replication of oriV-rep in E. coli was restored when additional rep was provided in trans, suggesting that the replication defect resulted from insufficient rep expression from its natural promoter. A promoter deficiency in E. coli was confirmed by reporter gene assays, transcriptional start point mapping and mutation of the promoter recognition elements. Dissection of the pM3 mini-replicon, pMT2, showed that this replication deficiency in E. coli is suppressed by additional determinants from its par operon: ParB, which can be supplied in trans, and its target, the par operon promoter, required in cis to oriV-rep. We propose that ParB binding to its target either changes plasmid DNA and thus promoter conformation or by spreading or looping contacts RNAP at the rep promoter so that rep expression is sufficient to activate oriV.

AB - IncP-9 plasmids are common in Pseudomonas species and can be transferred to other Gram-negative eubacteria but tend not to be stably maintained outside their natural host genus. A 1.3 kb oriV-rep fragment from IncP-9 plasmid pM3 was sufficient for autonomous replication in Pseudomonas putida but not in Escherichia coli. Replication of oriV-rep in E. coli was restored when additional rep was provided in trans, suggesting that the replication defect resulted from insufficient rep expression from its natural promoter. A promoter deficiency in E. coli was confirmed by reporter gene assays, transcriptional start point mapping and mutation of the promoter recognition elements. Dissection of the pM3 mini-replicon, pMT2, showed that this replication deficiency in E. coli is suppressed by additional determinants from its par operon: ParB, which can be supplied in trans, and its target, the par operon promoter, required in cis to oriV-rep. We propose that ParB binding to its target either changes plasmid DNA and thus promoter conformation or by spreading or looping contacts RNAP at the rep promoter so that rep expression is sufficient to activate oriV.

UR - http://www.scopus.com/inward/record.url?scp=22644436536&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2958.2005.04732.x

DO - 10.1111/j.1365-2958.2005.04732.x

M3 - Article

VL - 57

SP - 819

EP - 833

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 3

ER -