A T cell-specific enhancer in the interleukin-3 locus is activated cooperatively by Oct and NFAT elements within a DNase I-hypersensitive site

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Authors

Colleges, School and Institutes

Abstract

Interleukin-3 (IL-3) is a cytokine that is expressed primarily in activated T cells. Here we identified an inducible T cell-specific enhancer 14 kb upstream of the IL-3 gene that responded to activation of T cell receptor signaling pathways. The IL-3 enhancer spanned an inducible cyclosporin A-sensitive DNase I-hypersensitive site found only in T cells. Four NFAT-like elements exist within the enhancer. The two most active NFAT-like elements were located at the center of the DNase I-hypersensitive site. One of these NFAT-like elements encompassed overlapping Oct- and NFATp/c-binding sites, which functioned in a highly synergistic manner. We suggest that the T cell-specific expression of the IL-3 gene is partly controlled through the enhancer by cooperation between Oct and NFAT family proteins.

Details

Original languageEnglish
Pages (from-to)175-85
Number of pages11
JournalImmunity
Volume6
Issue number2
Publication statusPublished - Feb 1997

Keywords

  • Base Sequence, Cyclosporine, DNA-Binding Proteins, Deoxyribonuclease I, Drug Synergism, Enhancer Elements, Genetic, Gene Expression Regulation, HeLa Cells, Homeodomain Proteins, Host Cell Factor C1, Humans, Interleukin-3, Jurkat Cells, Molecular Sequence Data, NFATC Transcription Factors, Nuclear Proteins, Octamer Transcription Factor-1, Octamer Transcription Factor-2, T-Lymphocytes, Transcription Factors